Cholvi Maria, Trelis María, Bueno-Marí Rubén, Khoubbane Messaoud, Gil Rosario, Marcilla Antonio, Moretti Riccardo
Area of Parasitology, Department of Pharmacy and Pharmaceutical Technology and Parasitology, Faculty of Pharmacy, Universitat de València, 46100 Valencia, Spain.
Joint Research Unit in Endocrinology, Nutrition and Clinical Dietetics, La Fe Health Research Institute, 46026 Valencia, Spain.
Insects. 2024 Mar 20;15(3):206. doi: 10.3390/insects15030206.
The emergence of insecticide resistance in arbovirus vectors is putting the focus on the development of new strategies for control. In this regard, the exploitation of endosymbionts is receiving increasing attention due to its demonstrated effectiveness in reducing the vectorial capacity of mosquitoes. Here, we describe the establishment of a naïve infection in a wild population of eastern Spain through a hybridization approach to obtain males capable of sterilizing wild females. The obtained lines were compared with the donor, ARP, previously artificially infected with Pip, regarding immature and adult survival, female fecundity, egg fertility, and level of induced sterility. Our results did not show significant differences between lines in any of the biological parameters analyzed, indicating the full suitability of the hybrids to be used as a control tool against . In particular, hybrid males induced 99.9% sterility in the eggs of wild females without the need for any preliminary treatment. Being harmless to non-target organisms and the environment, the use of this bacterium for the control of deserves further exploration. This is especially relevant in areas such as eastern Spain, where this mosquito species has recently spread and may represent a serious threat due to its competence as a vector for dengue, chikungunya, and Zika viruses.
虫媒病毒载体中杀虫剂抗性的出现，使得控制新策略的开发成为焦点。在这方面，由于共生菌在降低蚊子传播能力方面已被证明有效，对其利用正受到越来越多的关注。在此，我们描述了通过杂交方法在西班牙东部野生种群中建立原始感染，以获得能够使野生雌蚊绝育的雄蚊。将获得的品系与先前人工感染了Pip的供体ARP在幼虫和成虫存活率、雌蚊繁殖力、卵的受精率以及诱导不育水平方面进行比较。我们的结果表明，在分析的任何生物学参数中，品系之间均未显示出显著差异，这表明这些杂交种完全适合用作针对……的控制工具。特别是，杂交雄蚊无需任何预处理就能使野生雌蚊的卵产生99.9%的不育率。这种细菌对非靶标生物和环境无害，利用其进行……的控制值得进一步探索。这在西班牙东部等地区尤为重要，在这些地区，这种蚊子物种最近已经扩散，并且由于其作为登革热、基孔肯雅热和寨卡病毒载体的能力，可能构成严重威胁。
