Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Science, Guangzhou, Guangdong, China.
Key Laboratory of Tropical Disease Control of the Ministry of Education, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China.
Microbiol Spectr. 2022 Oct 26;10(5):e0263321. doi: 10.1128/spectrum.02633-21. Epub 2022 Jul 27.
is being developed as a biological tool to suppress mosquito populations and/or interfere with their transmitted viruses. Adult males with an artificial infection have been released, successfully yielding population suppression in multiple field trials. The main characteristic of the artificial -infected mosquitoes used in the suppression program is the lower vector competence than that in native infected/uninfected mosquitoes in horizontal and vertical transmission. Our previous studies have demonstrated that the Aedes albopictus HC line infected with a trio of strains exhibited almost complete blockade of dengue virus (DENV) and Zika virus (ZIKV) in horizontal and vertical transmission. However, the extent to which inhibits virus transovarial transmission is unknown since no studies have been performed to determine whether protects ovarian cells against viral infection. Here, we employed ovarian cells of the . GUA (a wild-type mosquito line superinfected with two native strains, AlbA and AlbB), HC, and GT lines (tetracycline-cured, -uninfected mosquitoes), which exhibit key traits, and compared them to better understand how inhibits ZIKV transovarial transmission. Our results showed that the infection rate of adult GT progeny was significantly higher than that of GUA progeny during the first and second gonotrophic cycles. In contrast, the infection rates of adult GT and GUA progeny were not significantly different during the third gonotrophic cycle. All examined adult HC progeny from three gonotrophic cycles were negative for ZIKV infection. A strong negative linear correlation existed between density and ZIKV load in the ovaries of mosquitoes. Although there is no obvious coexistence area in the ovaries for and ZIKV, host immune responses may play a role in blocking ZIKV expansion and maintenance in the ovaries of . These results will aid in understanding -ZIKV interactions in mosquitoes. Area-wide application of to suppress mosquito populations and their transmitted viruses has achieved success in multiple countries. However, the mass release of -infected male mosquitoes involves a potential risk of accidentally releasing fertile females. In this study, we employed ovarian cells of the GUA, HC, and GT lines, which exhibit key traits, and compared them to better understand how inhibits ZIKV transovarial transmission. Our results showed an almost complete blockade of ZIKV transmission in HC female mosquitoes. in natively infected GUA mosquitoes negative affected ZIKV, and this interference was shown by slightly lower loads than those in HC mosquitoes. Overall, our work helps show how blocks ZIKV expansion and maintenance in the ovaries of and aids in understanding -ZIKV interactions in mosquitoes.
正在被开发为一种生物工具，以抑制蚊子种群和/或干扰它们传播的病毒。已经释放了经过人工感染的雄性成虫，在多个现场试验中成功地实现了种群抑制。在抑制计划中使用的人工感染蚊子的主要特征是在水平和垂直传播方面，其媒介能力比本地感染/未感染的蚊子低。我们之前的研究表明，感染了三株 株系的白纹伊蚊 HC 系在水平和垂直传播中几乎完全阻断了登革热病毒（DENV）和寨卡病毒（ZIKV）。然而，由于尚未进行研究以确定 是否可以保护卵巢细胞免受病毒感染，因此尚不清楚 对病毒经卵传播的抑制程度。在这里，我们使用了卵巢细胞的 GUA（一种超感染了两种本地 株系 AlbA 和 AlbB 的野生型蚊子系），HC 和 GT 系（四环素治愈，未感染的蚊子），这些系具有关键特征，并对它们进行了比较，以更好地了解 如何抑制 ZIKV 的经卵传播。我们的结果表明，在第一和第二生殖周期中，GT 后代成虫的感染率明显高于 GUA 后代成虫的感染率。相反，在第三个生殖周期中，GT 和 GUA 后代成虫的感染率没有明显差异。在三个生殖周期中，所有检查的 HC 后代成虫均未感染 ZIKV。ZIKV 在蚊子卵巢中的载量与 的密度之间存在很强的负线性相关性。尽管在 和 ZIKV 的卵巢中没有明显的共存区域，但宿主免疫反应可能在 阻止 ZIKV 在卵巢中的扩张和维持中发挥作用。这些结果将有助于理解蚊子中的 -ZIKV 相互作用。 在多个国家，使用 来抑制蚊子种群及其传播的病毒已取得成功。然而，大量释放感染的雄性蚊子可能会意外释放出有生育能力的雌性蚊子。在这项研究中，我们使用了卵巢细胞的 GUA、HC 和 GT 系，这些系具有关键特征，并对它们进行了比较，以更好地了解 如何抑制 ZIKV 的经卵传播。我们的结果表明，HC 雌性蚊子几乎完全阻断了 ZIKV 的传播。 在天然感染的 GUA 蚊子中， 对 ZIKV 产生了负面影响，这一点通过比 HC 蚊子略低的载量来显示。总的来说，我们的工作有助于展示 如何阻断 ZIKV 在 卵巢中的扩张和维持，并有助于理解蚊子中的 -ZIKV 相互作用。
