Tanyitiku Mary Nkongho, Nicholas Graeme, Sullivan Jon J, Petcheu Igor C Njombissie, On Stephen L W
Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647, New Zealand.
Department of Pest-Management and Conservation, Lincoln University, Lincoln 7647, New Zealand.
Pathogens. 2024 Feb 26;13(3):204. doi: 10.3390/pathogens13030204.
Land snails are considered a delicacy in many countries in Europe and sub-Saharan Africa. However, the interaction of microbial pathogens with land snails may present a public health threat when handling and/or consuming snails. This study examines the survival of in edible land snails in a model system.
Well-studied Shigatoxigenic (STEC) and non-STEC strains were compared. Mature spp. were experimentally fed with -inoculated oats for 48 h. The snail feces after inoculation were periodically sampled and cultured for a 30-day period and subjected to microbiological analyses.
The average rate of decline of the non-STEC strain CSH-62 in the feces of live snails was significantly ( < 0.05) faster than that of STEC ERL 06-2503. In addition, the viable population of ERL 06-2503 significantly ( < 0.05) persisted for a longer time in the intestine of land snails than CSH-62.
The results showed that the viable population of the strains examined demonstrated first-order kinetics, and their survival (CFU/mL) appeared significantly ( < 0.05) dependent on the pathotype. In addition, the continuous enumeration of in snail faeces indicated that land snails could serve as a mode of transmission of microbial pathogens to susceptible hosts, including humans. Further research is recommended to better quantify the direct and indirect health risks of pathogen transmission by edible snails to humans.
在欧洲和撒哈拉以南非洲的许多国家，陆地蜗牛被视为美味佳肴。然而，在处理和/或食用蜗牛时，微生物病原体与陆地蜗牛的相互作用可能会对公众健康构成威胁。本研究在一个模型系统中检测了可食用陆地蜗牛体内[病原体名称未给出]的存活情况。
比较了经过充分研究的产志贺毒素大肠杆菌（STEC）和非STEC菌株。用接种了[病原体名称未给出]的燕麦对成熟的[蜗牛种类未给出]进行48小时的实验性投喂。对接种后的蜗牛粪便进行定期采样，并在30天内进行培养，然后进行微生物学分析。
活蜗牛粪便中，非STEC菌株CSH - 62的平均数量下降速率显著（P < 0.05）快于STEC菌株ERL 06 - 2503。此外，STEC菌株ERL 06 - 2503在陆地蜗牛肠道中的活菌数量显著（P < 0.05）比CSH - 62持续更长时间。
结果表明，所检测的[病原体名称未给出]菌株的活菌数量呈现一级动力学，并且它们的存活情况（CFU/mL）似乎显著（P < 0.05）取决于[病原体名称未给出]的致病型。此外，对蜗牛粪便中[病原体名称未给出]的持续计数表明，陆地蜗牛可能成为微生物病原体向包括人类在内的易感宿主传播的一种途径。建议进一步开展研究，以更好地量化可食用蜗牛向人类传播病原体的直接和间接健康风险。
