蛋白质组学分析揭示的Y-4中钯（II）的还原机制
The Pd (II) Reduction Mechanisms in Y-4 Revealed by Proteomic Analysis.
作者信息
Chen Yuan, Wang Jiaxing, Chen Daidi, Wang Boxi, Wu Jinchuan, Liu Rongrong, Li Qingxin
机构信息
Guangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, China.
Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Institute of Environmental Research at Greater Bay Area, Ministry of Education, Guangzhou University, Guangzhou 510006, China.
出版信息
Nanomaterials (Basel). 2024 Mar 12;14(6):512. doi: 10.3390/nano14060512.
Many studies have been conducted on the microbial reduction of Pd (II) to palladium nanoparticles (Pd-NPs) due to the environmental friendliness, low cost, and the decreased toxicity of Pd (II) ions. In this study, we investigate the reduction mechanism of Pd (II) by Y-4 through proteomics. The data are available via ProteomeXchange with identifier PXD049711. Our results revealed that Y-4 may use the endogenous electron donor (NAD(P)H) generated by nirB, tdh, and fabG and reductase to reduce Pd (II) to Pd-NPs. The expression levels of fabG, tdh, gudB, and rocG that generate NAD(P)H were further increased, and the number of reduced Pd-NPs was further increased with the exogenous electron donor sodium formate. Endogenous electron mediators such as quinones and flavins in Y-4 can further enhance Pd (II) reduction. The findings provided invaluable information regarding the reduction mechanism of Pd (II) by Y-4 at the proteome level.
由于环境友好、成本低以及钯（II）离子毒性降低，许多研究围绕微生物将钯（II）还原为钯纳米颗粒（Pd-NPs）展开。在本研究中，我们通过蛋白质组学研究Y-4对钯（II）的还原机制。数据可通过ProteomeXchange获取，标识符为PXD049711。我们的结果表明，Y-4可能利用由nirB、tdh和fabG以及还原酶产生的内源性电子供体（NAD(P)H）将钯（II）还原为Pd-NPs。产生NAD(P)H的fabG、tdh、gudB和rocG的表达水平进一步提高，并且随着外源性电子供体甲酸钠的加入，还原的Pd-NPs数量进一步增加。Y-4中的内源性电子介质如醌和黄素可进一步增强钯（II）的还原。这些发现为Y-4在蛋白质组水平上对钯（II）的还原机制提供了宝贵信息。
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