• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蛋白质组学分析揭示的Y-4中钯（II）的还原机制

The Pd (II) Reduction Mechanisms in Y-4 Revealed by Proteomic Analysis.

作者信息

Chen Yuan, Wang Jiaxing, Chen Daidi, Wang Boxi, Wu Jinchuan, Liu Rongrong, Li Qingxin

机构信息

Guangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, China.

Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Institute of Environmental Research at Greater Bay Area, Ministry of Education, Guangzhou University, Guangzhou 510006, China.

出版信息

Nanomaterials (Basel). 2024 Mar 12;14(6):512. doi: 10.3390/nano14060512.

DOI:10.3390/nano14060512
PMID:38535660
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10976171/
Abstract

Many studies have been conducted on the microbial reduction of Pd (II) to palladium nanoparticles (Pd-NPs) due to the environmental friendliness, low cost, and the decreased toxicity of Pd (II) ions. In this study, we investigate the reduction mechanism of Pd (II) by Y-4 through proteomics. The data are available via ProteomeXchange with identifier PXD049711. Our results revealed that Y-4 may use the endogenous electron donor (NAD(P)H) generated by nirB, tdh, and fabG and reductase to reduce Pd (II) to Pd-NPs. The expression levels of fabG, tdh, gudB, and rocG that generate NAD(P)H were further increased, and the number of reduced Pd-NPs was further increased with the exogenous electron donor sodium formate. Endogenous electron mediators such as quinones and flavins in Y-4 can further enhance Pd (II) reduction. The findings provided invaluable information regarding the reduction mechanism of Pd (II) by Y-4 at the proteome level.

摘要

由于环境友好、成本低以及钯（II）离子毒性降低，许多研究围绕微生物将钯（II）还原为钯纳米颗粒（Pd-NPs）展开。在本研究中，我们通过蛋白质组学研究Y-4对钯（II）的还原机制。数据可通过ProteomeXchange获取，标识符为PXD049711。我们的结果表明，Y-4可能利用由nirB、tdh和fabG以及还原酶产生的内源性电子供体（NAD(P)H）将钯（II）还原为Pd-NPs。产生NAD(P)H的fabG、tdh、gudB和rocG的表达水平进一步提高，并且随着外源性电子供体甲酸钠的加入，还原的Pd-NPs数量进一步增加。Y-4中的内源性电子介质如醌和黄素可进一步增强钯（II）的还原。这些发现为Y-4在蛋白质组水平上对钯（II）的还原机制提供了宝贵信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/595e8a58390c/nanomaterials-14-00512-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/fad259b74011/nanomaterials-14-00512-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/1a0452151364/nanomaterials-14-00512-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/f8752f5d9984/nanomaterials-14-00512-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/66fdb1b82288/nanomaterials-14-00512-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/e298355a0248/nanomaterials-14-00512-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/1ee2b70e3f1f/nanomaterials-14-00512-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/9ecc714bdefc/nanomaterials-14-00512-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/595e8a58390c/nanomaterials-14-00512-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/fad259b74011/nanomaterials-14-00512-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/1a0452151364/nanomaterials-14-00512-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/f8752f5d9984/nanomaterials-14-00512-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/66fdb1b82288/nanomaterials-14-00512-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/e298355a0248/nanomaterials-14-00512-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/1ee2b70e3f1f/nanomaterials-14-00512-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/9ecc714bdefc/nanomaterials-14-00512-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ab/10976171/595e8a58390c/nanomaterials-14-00512-g008.jpg

相似文献

1
The Pd (II) Reduction Mechanisms in Y-4 Revealed by Proteomic Analysis.蛋白质组学分析揭示的Y-4中钯（II）的还原机制
Nanomaterials (Basel). 2024 Mar 12;14(6):512. doi: 10.3390/nano14060512.
2
The effect of biotic and abiotic environmental factors on Pd(II) adsorption and reduction by Bacillus megaterium Y-4.生物和非生物环境因素对巨大芽孢杆菌Y-4吸附和还原Pd(II)的影响。
Chemosphere. 2019 Apr;220:1058-1066. doi: 10.1016/j.chemosphere.2019.01.011. Epub 2019 Jan 4.
3
The effect of biotic and abiotic environmental factors on Pd(II) adsorption and reduction by Bacillus wiedmannii MSM.生物和非生物环境因素对巨大芽孢杆菌 MSM 吸附和还原 Pd(II)的影响。
Ecotoxicol Environ Saf. 2018 Oct 30;162:546-553. doi: 10.1016/j.ecoenv.2018.07.043. Epub 2018 Jul 18.
4
Intra/extracellular electron transfer for aerobic denitrification mediated by in-situ biosynthesis palladium nanoparticles.原位生物合成钯纳米粒子介导的好氧反硝化中的胞内/胞外电子传递。
Water Res. 2021 Feb 1;189:116612. doi: 10.1016/j.watres.2020.116612. Epub 2020 Nov 6.
5
Triclosan detoxification through dechlorination and oxidation via microbial Pd-NPs under aerobic conditions.在有氧条件下，通过微生物 Pd-NPs 的脱氯和氧化作用来解毒三氯生。
Chemosphere. 2022 Jan;286(Pt 3):131836. doi: 10.1016/j.chemosphere.2021.131836. Epub 2021 Aug 10.
6
D-mannitol production by resting state whole cell biotrans-formation of D-fructose by heterologous mannitol and formate dehydrogenase gene expression in Bacillus megaterium.通过在巨大芽孢杆菌中异源表达甘露醇脱氢酶和甲酸脱氢酶基因，利用静止状态的全细胞生物转化D-果糖生产D-甘露醇。
Biotechnol J. 2007 Nov;2(11):1408-16. doi: 10.1002/biot.200700055.
7
Novel Microbial Palladium Nanoparticles with a High Photothermal Effect for Antibacterial Applications.具有高光热效应的新型微生物钯纳米颗粒在抗菌应用中的研究
ACS Omega. 2022 Dec 22;8(1):1534-1541. doi: 10.1021/acsomega.2c07037. eCollection 2023 Jan 10.
8
Difference in toxicity of Pd (II) and mechanism of action before and after reduction by Bacillus wiedmannii MSM.MSM 菌还原前后 Pd（II）的毒性差异及其作用机制。
Environ Sci Pollut Res Int. 2022 Jan;29(2):1824-1835. doi: 10.1007/s11356-021-15736-y. Epub 2021 Aug 6.
9
Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1.黄素介导革兰氏阳性巨大芽孢杆菌菌株LLD-1中的细胞外电子转移。
Bioelectrochemistry. 2018 Feb;119:196-202. doi: 10.1016/j.bioelechem.2017.10.005. Epub 2017 Oct 16.
10
Electron transfer involved in bio-Pd (0) synthesis by Citrobacter freundii at different growth phases.不同生长阶段弗氏柠檬酸杆菌生物合成零价钯中的电子传递。
Ecotoxicol Environ Saf. 2020 Mar 1;190:110124. doi: 10.1016/j.ecoenv.2019.110124. Epub 2019 Dec 26.

本文引用的文献

1
Coupling of selenate reduction and pyrrhotite oxidation by indigenous microbial consortium in natural aquifer.天然含水层中本地微生物群落对硒酸盐还原与磁黄铁矿氧化的耦合作用。
Water Res. 2023 Jun 30;238:119987. doi: 10.1016/j.watres.2023.119987. Epub 2023 Apr 20.
2
The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences.PRIDE 数据库资源在 2022 年：一个基于质谱的蛋白质组学证据的中心。
Nucleic Acids Res. 2022 Jan 7;50(D1):D543-D552. doi: 10.1093/nar/gkab1038.
3
Reduction and removal of Cr(VI) in water using biosynthesized palladium nanoparticles loaded Shewanella oneidensis MR-1.
利用合成的负载于希瓦氏菌属的钯纳米粒子还原和去除水中的六价铬。
Sci Total Environ. 2022 Jan 20;805:150336. doi: 10.1016/j.scitotenv.2021.150336. Epub 2021 Sep 15.
4
Difference in toxicity of Pd (II) and mechanism of action before and after reduction by Bacillus wiedmannii MSM.MSM 菌还原前后 Pd（II）的毒性差异及其作用机制。
Environ Sci Pollut Res Int. 2022 Jan;29(2):1824-1835. doi: 10.1007/s11356-021-15736-y. Epub 2021 Aug 6.
5
The effect of biotic and abiotic environmental factors on Pd(II) adsorption and reduction by Bacillus megaterium Y-4.生物和非生物环境因素对巨大芽孢杆菌Y-4吸附和还原Pd(II)的影响。
Chemosphere. 2019 Apr;220:1058-1066. doi: 10.1016/j.chemosphere.2019.01.011. Epub 2019 Jan 4.
6
Recycling of Pd(0) catalysts by magnetic nanocomposites-microbial extracellular polymeric substances@FeO.磁性纳米复合材料-微生物胞外聚合物@FeO 回收 Pd(0)催化剂。
J Environ Manage. 2021 Feb 15;280:111834. doi: 10.1016/j.jenvman.2020.111834. Epub 2020 Dec 23.
7
Identification of genes responsible for reducing palladium ion in Escherichia coli.鉴定大肠杆菌中降低钯离子的基因。
J Biotechnol. 2020 Dec 20;324:7-10. doi: 10.1016/j.jbiotec.2020.09.015. Epub 2020 Sep 22.
8
Insights into palladium nanoparticles produced by Shewanella oneidensis MR-1: Roles of NADH dehydrogenases and hydrogenases.对希瓦氏菌MR-1产生的钯纳米颗粒的见解：NADH脱氢酶和氢化酶的作用。
Environ Res. 2020 Dec;191:110196. doi: 10.1016/j.envres.2020.110196. Epub 2020 Sep 10.
9
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma.基于基因组与蛋白质组联合分析的肺腺癌治疗靶点研究
Cell. 2020 Jul 9;182(1):200-225.e35. doi: 10.1016/j.cell.2020.06.013.
10
Proteomic analysis of the reduction and resistance mechanisms of Shewanella oneidensis MR-1 under long-term hexavalent chromium stress.长期六价铬胁迫下希瓦氏菌 MR-1 的还原和抗性机制的蛋白质组学分析。
Environ Int. 2019 Jun;127:94-102. doi: 10.1016/j.envint.2019.03.016. Epub 2019 Mar 23.