Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Environ Int. 2019 Jun;127:94-102. doi: 10.1016/j.envint.2019.03.016. Epub 2019 Mar 23.
Hexavalent chromium [Cr(VI)] is a priority heavy metal pollutant causing a series of environmental issues, and bio-reduction of Cr(VI) to trivalent chromium can remarkably decrease the environmental risk of Cr(VI). The reduction and resistance abilities of microorganisms to Cr(VI) can be dramatically improved by acclimatization. In the present study, we collected Shewanella oneidensis MR-1 from a 120-day acclimatization by increasing Cr(VI) concentration in the culture media to investigate its adaptation mechanisms under long-term Cr(VI) stress at the proteome level. Tandem mass tag-based quantitative proteomic analysis was performed to study the differences between 9 collected samples. A total of 2500 proteins were quantified from 2723 identified protein groups. Bioinformatics analysis showed that the differentially expressed proteins after the 120-day Cr(VI) acclimatization were mostly related to flagellar assembly, ribosomes, transport, sulfur metabolism, and energy metabolism. The findings of this study present novel insights into the molecular mechanisms for the reduction and resistance of S. oneidensis MR-1 responding to long-term Cr(VI) stress at the proteome level.
六价铬[Cr(VI)]是一种优先重金属污染物，会引发一系列环境问题，而将六价铬还原为三价铬可以显著降低 Cr(VI)的环境风险。通过驯化可以显著提高微生物对 Cr(VI)的还原和抗性能力。在本研究中，我们从培养物中 Cr(VI)浓度增加 120 天的驯化中收集 Shewanella oneidensis MR-1，以在蛋白质组水平上研究其在长期 Cr(VI)胁迫下的适应机制。采用串联质量标签定量蛋白质组学分析来研究 9 个收集样本之间的差异。从 2723 个鉴定的蛋白质组中定量了 2500 种蛋白质。生物信息学分析表明，经过 120 天 Cr(VI)驯化后差异表达的蛋白质主要与鞭毛组装、核糖体、运输、硫代谢和能量代谢有关。本研究的结果为 S. oneidensis MR-1 在蛋白质组水平上对长期 Cr(VI)胁迫的还原和抗性的分子机制提供了新的见解。
