Velasco Carlos, Ortega Javier, Gómez-Buendía Alberto, Grau Anna, López Marisol, Álvarez Julio, Romero Beatriz, de Juan Lucía, Bezos Javier
VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain.
Department of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, Spain.
Vet Sci. 2024 Feb 28;11(3):105. doi: 10.3390/vetsci11030105.
Paratuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goats subjected to the comparative intradermal tuberculin test (CITT) may experience a booster effect on their antibody titer and a potential enhancement to the sensitivity of humoral techniques for tuberculosis, in the present study we aimed to evaluate this diagnostic strategy on the humoral diagnosis of PTB in serum and milk samples collected from a caprine herd that was TB free and PTB infected. The results from 120 goats indicated a significant increase ( < 0.001) in the quantitative response detected using an ELISA technique, conducted using serum and milk samples taken 15 and 30 days after performing a CITT (day 0 of the study); although, it did not translate into a significant increase in the number of reactors during any of the testing events (0, 3,15, 30 and 60 days post-CITT). Additionally, the number of ELISA-positive animals was higher for the serum versus the milk samples at both 15 and 30 days post-CITT. The increase in the quantitative ELISA result suggested a diagnostic strategy that maximizes ELISA sensitivity, mainly using serum samples, in PTB-infected herds; although, it may depend on individual differences and the interpretation criteria.
副结核病（PTB）和结核病（TB）是两种对家养反刍动物具有严重经济和健康影响的分枝杆菌病。PTB的生前诊断受到诸多因素的阻碍，其中包括所有现有诊断技术的敏感性有限。由于接受比较皮内结核菌素试验（CITT）的TB感染山羊可能会出现抗体滴度的增强效应以及体液技术对结核病敏感性的潜在提高，在本研究中，我们旨在评估这种诊断策略对从一个无TB且感染PTB的山羊群中采集的血清和乳样进行PTB体液诊断的效果。120只山羊的结果表明，在进行CITT（研究第0天）后15天和30天采集血清和乳样，使用ELISA技术检测到的定量反应有显著增加（<0.001）；尽管如此，在任何测试事件（CITT后0、3、15、30和60天）期间反应动物的数量均未出现显著增加。此外，在CITT后15天和30天，血清样本的ELISA阳性动物数量均高于乳样。ELISA定量结果的增加表明了一种诊断策略，即在PTB感染的畜群中，主要使用血清样本可使ELISA敏感性最大化；不过，这可能取决于个体差异和解释标准。
