Giri Pravin Shankar, Rath Subha Narayan
Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, 502284 Telangana, India.
ACS Appl Bio Mater. 2024 Apr 15;7(4):2413-2422. doi: 10.1021/acsabm.4c00066. Epub 2024 Mar 27.
The interaction between biomaterials and the immune system plays a pivotal role in determining the success or failure of implantable devices. Macrophages, as key orchestrators of immune responses, exhibit diverse reactions that influence tissue integration or lead to implant failure. This study focuses on unraveling the intricate relationship between macrophage phenotypes and biomaterials, specifically hydrogels, by employing THP-1 cells as a model. Through a comprehensive investigation using polysaccharide, polymer, and protein-based hydrogels, our research sheds light on how the properties of hydrogels influence macrophage polarization. Phenotypic observations, biochemical assays, surface marker expression, and gene expression profiles collectively demonstrate the differential macrophage polarization abilities of polysaccharide-, polymer-, and protein-based hydrogels. Moreover, our indirect coculture studies reveal that hydrogels fostering M2 polarization exhibit exceptional wound-healing capabilities. These findings highlight the crucial role of the hydrogel microenvironment in adjusting macrophage polarization, offering a fresh avenue for refining biomaterials to bolster advantageous immune responses and improve tissue integration. This research contributes valuable insights for designing biomaterials with tailored properties that can guide macrophage behavior, ultimately improving the overall success of implantable devices.
生物材料与免疫系统之间的相互作用在决定可植入设备的成败方面起着关键作用。巨噬细胞作为免疫反应的关键协调者，表现出多种反应，这些反应会影响组织整合或导致植入失败。本研究通过采用THP-1细胞作为模型，专注于揭示巨噬细胞表型与生物材料（特别是水凝胶）之间的复杂关系。通过使用基于多糖、聚合物和蛋白质的水凝胶进行全面研究，我们的研究揭示了水凝胶的特性如何影响巨噬细胞极化。表型观察、生化分析、表面标志物表达和基因表达谱共同证明了基于多糖、聚合物和蛋白质的水凝胶具有不同的巨噬细胞极化能力。此外，我们的间接共培养研究表明，促进M2极化的水凝胶具有出色的伤口愈合能力。这些发现突出了水凝胶微环境在调节巨噬细胞极化中的关键作用，为改进生物材料以增强有利的免疫反应和改善组织整合提供了新途径。这项研究为设计具有定制特性的生物材料提供了有价值的见解，这些特性可以引导巨噬细胞行为，最终提高可植入设备的整体成功率。
