Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
Photobiomodul Photomed Laser Surg. 2024 Apr;42(4):314-320. doi: 10.1089/photob.2023.0194. Epub 2024 Mar 27.
, a nosocomial pathogen, poses a major public health problem due to generating resistance to several antimicrobial agents. Antimicrobial photodynamic inactivation (APDI) employs a nontoxic dye as a photosensitizer (PS) and light to produce reactive oxygen species that destroy bacterial cells. The intracellular concentration of PS could be affected by factors such as the function of efflux pumps to emit PS from the cytosol. To evaluate the augmentation effect of an efflux pump inhibitor, verapamil, three multidrug-resistant were subjected to APDI by erythrosine B (EB). The combination of EB and verapamil along with irradiation at 530 nm induced a lethal effect and more than 3 log colony-forming unit reduction to all strains in planktonic state. In contrast, EB and irradiation alone could produce only a sublethal effect on two of the strains. These data suggest that verapamil increases the intracellular concentration of EB, which potentiates the lethal efficacy of APDI. Verapamil could be applied with EB and green light to improve their antimicrobial efficacy against -localized infections.
铜绿假单胞菌是一种医院病原体，由于对几种抗菌药物产生耐药性，因此成为一个主要的公共卫生问题。抗菌光动力灭活（APDI）采用无毒染料作为光敏剂（PS）和光来产生破坏细菌细胞的活性氧。PS 的细胞内浓度可能受到外排泵功能等因素的影响，这些因素可将 PS 从细胞质中排出。为了评估外排泵抑制剂维拉帕米的增强作用，用血卟啉单甲醚（EB）对三种多药耐药的铜绿假单胞菌进行了 APDI 处理。EB 与维拉帕米联合 530nm 光照诱导所有浮游状态的菌株产生致死效应和超过 3 对数集落形成单位减少。相比之下，EB 和光照单独处理只能对其中两种菌株产生亚致死效应。这些数据表明，维拉帕米增加了 EB 的细胞内浓度，从而增强了 APDI 的致死效果。维拉帕米可与 EB 和绿光一起应用，以提高它们对局部感染的抗菌效果。
