人乳中多酚的快速、经济、准确的微量分析方法：转化开发。

Polyphenol analysis in human milk by a rapid, cost-effective, and accurate micromethod: translational development.

机构信息

Universidad Nacional de Córdoba, Facultad de Ciencias Médicas, Escuela de Fonoaudiología, Ciudad de Córdoba, Córdoba, Argentina.

Montpellier Interdisciplinary Center on Sustainable Agri-food Systems (MoISA), University of Montpellier, CIRAD, CIHEAM-IAMM, INRAE, Institut Agro, IRD, Montpellier, France.

出版信息

Rev Paul Pediatr. 2024 Mar 25;42:e2023186. doi: 10.1590/1984-0462/2024/42/2023186. eCollection 2024.

DOI:10.1590/1984-0462/2024/42/2023186

PMID:38537035

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10962630/

Abstract

OBJECTIVE

To develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk (HM) volumes.

METHODS

Acid and alkaline extractions were compared using two dyes: Folin-Ciocalteu and Fast Blue BB. Linearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 HM samples and compared using the Kruskal-Wallis H test (p<0.05). The best technique was applied to 284 HM samples to determine their polyphenolic content and its association with maternal diet by multifactorial linear regression.

RESULTS

Acidic extraction successfully recovered the gallic acid reference standard, whereas alkaline extraction overestimated it. Calibration curves for all methods were linear (R2>0.96) up to 500 mg/L. All bicarbonate-based Folin-Ciocalteu methods assayed were stable and repeatable, whereas Fast Blue BB-based variants were not. HM polyphenols (mean=94.68 mg/L) positively correlated to the dietary intake of hydroxycinnamic acids, the most consumed polyphenolic family in this population.

CONCLUSIONS

A bicarbonate-based Folin-Ciocalteu micromethod allowed the accurate determination of polyphenols in HM, which might be useful for translational research settings and HM banks.

摘要

目的

开发一种快速分析多酚的方法，这些多酚是针对新生儿氧化应激的潜在活性抗氧化剂，从小量人乳（HM）中提取。

方法

使用两种染料：福林-考尔太乌和 Fast Blue BB 比较酸提取和碱提取。在 14 个人乳样本中评估线性、灵敏度、回收率、多酚含量、精密度和稳定性，并使用 Kruskal-Wallis H 检验（p<0.05）进行比较。最佳技术应用于 284 个人乳样本，通过多因素线性回归确定其多酚含量及其与母体饮食的关系。

结果

酸性提取成功回收了没食子酸参考标准，而碱性提取则高估了它。所有方法的校准曲线在 500 mg/L 以下均呈线性（R2>0.96）。所有基于碳酸氢盐的福林-考尔太乌方法均稳定且可重复，而基于 Fast Blue BB 的变体则不稳定。HM 中的多酚（平均值=94.68 mg/L）与人群中最消耗的多酚家族羟基肉桂酸的饮食摄入量呈正相关。