Miranda A R, Albrecht C, Soria E A
Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Córdoba, CONICET, FCM (Enrique Barros, Ciudad Universitaria, CP 5014), Córdoba, Argentina.
Instituto Nacional del Cáncer, Ministerio de Salud de la Nación (Av. Julio A. Roca 781 10°, CABA), Buenos Aires, Argentina. Autor de correspondencia.
Rev Fac Cien Med Univ Nac Cordoba. 2017;74(2):119-125.
Biomedical potential of polyphenols lies in their ability to modulate redox balance and the mechanisms involved in the development of chronic noncommunicable diseases.
The aim of this study was to determine the concentration of total polyphenols in different murine organs by assaying analytical techniques of Folin Ciocalteu (FC) and Fast Blue BB (FBBB).
Balb/c female mice (n≥3) received for 15 days 100 mg/kg/d of extract of Lantana grisebachii (LG), Aspidosperma quebracho-blanco (AQB) or Ilex paraguariensis (IP) and control group (treated with water without extract). Polyphenolic concentrations were measured in telencephalon, diencephalon, midbrain, brainstem, cerebellum, spleen, thymus and cardiopulmonary tissue by FC and FBBB methods. Results were compared by ANOVA followed by Tukey test (p<0.05).
FBBB method reported higher detections than FC (4.5 fold in telencephalon, 8.4 in midbrain, 5 in brainstem, 7.2 in spleen, 68.5 in thymus and 4 in cardiopulmonary tissue). Regarding the treatments, the group that received AQB showed to have increased polyphenolic bioavailability in brainstem (p<0.02). With FBBB, a decrease on thymic polyphenol content after treatment with IP was detected (p<0.005). In cerebellum of the groups treated with IP and telencephalon of the control group showed significant differences when these were analyzed with FC (p<0.05, p<0.0035 respectively).
FBBB method showed higher estimations of polyphenolic bioavailability than FC, and this could be related to higher specificity of the technique to react with phenolic compounds.
多酚类物质的生物医学潜力在于它们调节氧化还原平衡的能力以及参与慢性非传染性疾病发展的机制。
本研究的目的是通过测定福林-西奥尔特（FC）法和固蓝BB（FBBB）法来确定不同小鼠器官中总多酚的浓度。
将雌性Balb/c小鼠（n≥3）分为三组，连续15天每天给予100mg/kg/d的灰团花提取物（LG）、白坚木提取物（AQB）或巴拉圭冬青提取物（IP），对照组给予不含提取物的水。采用FC法和FBBB法测定端脑、间脑、中脑、脑干、小脑、脾脏、胸腺和心肺组织中的多酚浓度。结果通过方差分析和Tukey检验进行比较（p<0.05）。
FBBB法的检测结果高于FC法（端脑高4.5倍，中脑高8.4倍，脑干高5倍，脾脏高7.2倍，胸腺高68.5倍，心肺组织高4倍）。在处理方面，接受AQB的组在脑干中的多酚生物利用度有所增加（p<0.02）。使用FBBB法检测到，用IP处理后胸腺中的多酚含量降低（p<0.005）。当用FC法分析时，IP处理组的小脑和对照组的端脑存在显著差异（分别为p<0.05，p<0.0035）。
FBBB法对多酚生物利用度的估计高于FC法，这可能与该技术与酚类化合物反应的更高特异性有关。
