Key Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei, 230009, PR China; School of Materials Science and Engineering, Hefei University of Technology, Hefei, 230009, PR China.
Key Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei, 230009, PR China; School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, 230009, PR China.
Talanta. 2024 Jul 1;274:125986. doi: 10.1016/j.talanta.2024.125986. Epub 2024 Mar 26.
The outbreak of novel coronavirus pneumonia (COVID-19) in 2019 has garnered widespread attention. The virus exhibits high contagiousness, and in certain cases, it can lead to recurrent infections. Therefore, it is imperative to develop portable, sensitive, and accurate sensors to promptly detect infected individuals, control the virus's transmission, and determine suitable treatment strategies. In this study, we proposed a magnetically-assisted method employing CFO@CS-Au MNP as the substrate material, which was functionalized with human angiotensin-converting enzyme (ACE2) for efficient capture of SARS-CoV-2 spike protein in solution. Subsequently, the captured protein was sensitively detected through differential pulse voltammetry (DPV) electrical analysis. The linear detection range of the labeled GCE/MNP/GA/ACE2/BSA electrochemical sensor is from 1 pg/mL to 10 μg/mL, with a minimum detection limit of 0.15 pg/mL. Furthermore, the fabricated GCE/MNP/GA/ACE2/BSA sensor achieved satisfactory recoveries of SARS-CoV-2 spike protein in saliva and nasal swab samples within 10 min. These results indicate that this magnetically-assisted biosensor has established a solid foundation for the swift on-site detection of COVID-19.
2019 年爆发的新型冠状病毒肺炎（COVID-19）引起了广泛关注。该病毒具有很强的传染性，在某些情况下，它会导致反复感染。因此，开发便携式、灵敏、准确的传感器来及时检测感染个体、控制病毒传播以及确定合适的治疗策略至关重要。在本研究中，我们提出了一种磁辅助方法，使用 CFO@CS-Au MNP 作为基底材料，其功能化有人血管紧张素转换酶（ACE2），可有效捕获溶液中的 SARS-CoV-2 刺突蛋白。随后，通过差分脉冲伏安法（DPV）进行灵敏检测。标记的 GCE/MNP/GA/ACE2/BSA 电化学传感器的线性检测范围为 1 pg/mL 至 10 μg/mL，检测限低至 0.15 pg/mL。此外，该制备的 GCE/MNP/GA/ACE2/BSA 传感器在 10 分钟内实现了唾液和鼻腔拭子样本中 SARS-CoV-2 刺突蛋白的令人满意的回收率。这些结果表明，这种磁辅助生物传感器为 COVID-19 的快速现场检测奠定了坚实的基础。
