Tachibana Y, Kanbe K, Mizutani T
Nucleic Acids Symp Ser. 1985(16):217-20.
Study by chemical modification of Ser, Arg, His residues and sulfhydryl groups on bovine seryl-tRNA synthetase showed that Ser residues appeared to be unnecessary for the recognition mechanism, but Arg and His residues were essential. It was considered that different sulfhydryl groups related with each recognition of tRNA and ATP. Poly-arginine inhibited the interaction between serine tRNA and SerRS. The CD spectra of a mixture of serine tRNA and poly-arginine indicated that higher-order structure of tRNA changed. Furthermore, the Km and Vmax values of bovine serine isoacceptor, yeast serine tRNA and E. coli serine tRNA for bovine SerRS examined and it was discussed the differences of those base sequences.
通过对牛丝氨酸 - tRNA合成酶上的丝氨酸（Ser）、精氨酸（Arg）、组氨酸（His）残基以及巯基进行化学修饰研究表明，丝氨酸残基对于识别机制似乎并非必需，但精氨酸和组氨酸残基至关重要。据认为，不同的巯基与tRNA和ATP的各自识别相关。聚精氨酸抑制丝氨酸tRNA与丝氨酸 - tRNA合成酶（SerRS）之间的相互作用。丝氨酸tRNA与聚精氨酸混合物的圆二色光谱表明tRNA的高级结构发生了变化。此外，还检测了牛丝氨酸同工受体、酵母丝氨酸tRNA和大肠杆菌丝氨酸tRNA对牛SerRS的米氏常数（Km）和最大反应速度（Vmax）值，并讨论了它们碱基序列的差异。
