Department of Gynecology, 3201 Hospital, HanZhong, China.
Department of Obstetrics, Laoling People's Hospital, Dezhou, China.
Chem Biol Drug Des. 2024 Mar;103(3):e14459. doi: 10.1111/cbdd.14459.
Diosgenin, a natural steroidal sapogenin, has recently attracted a high amount of attention, as an effective anticancer agent in ovarian cancer. However, diosgenin mediated anticancer impacts are still not completely understood. Thus, the present study evaluated the effect of diosgenin on the proliferation, apoptosis, and metastasis of ovarian cancer cells. OVCAR-3 and SKOV-3 cells were treated with diosgenin, cellular viability was assessed by MTT assay and apoptosis was measured by ELISA and evaluated the protein expression levels of apoptotic markers through western blotting. Cell migration was examined by measuring the mRNA levels of genes involved in the cell invasion. The protein expression levels of main components of PI3K signaling were evaluated via western blotting. Diosgenin led to significant inhibition of cellular proliferation in a dose-dependent manner. It also induced apoptosis through upregulating pro-apoptotic markers and downregulating antiapoptotic mediators. In addition, OVCAR-3 cells exposure to diosgenin decreased cell migration and invasion. More importantly, diosgenin downregulated the expression levels of main proteins in PI3K signaling including PI3K, Akt, mTOR, and GSK3. Diosgenin inhibited the proliferation and migration of OVCAR-3 ovarian cancer cells and induced apoptosis, which may be mediated by targeting PI3K signaling.
薯蓣皂苷元，一种天然甾体皂素，最近受到了广泛关注，作为卵巢癌的一种有效抗癌药物。然而，薯蓣皂苷元介导的抗癌作用仍不完全清楚。因此，本研究评估了薯蓣皂苷元对卵巢癌细胞增殖、凋亡和转移的影响。用薯蓣皂苷元处理 OVCAR-3 和 SKOV-3 细胞，通过 MTT 法评估细胞活力，通过 ELISA 测量细胞凋亡，并通过 Western blot 评估凋亡标志物的蛋白表达水平。通过测量参与细胞侵袭的基因的 mRNA 水平来检测细胞迁移。通过 Western blot 评估 PI3K 信号主要成分的蛋白表达水平。薯蓣皂苷元以剂量依赖性方式显著抑制细胞增殖。它还通过上调促凋亡标志物和下调抗凋亡介质来诱导细胞凋亡。此外，薯蓣皂苷元处理 OVCAR-3 细胞可降低细胞迁移和侵袭。更重要的是，薯蓣皂苷元下调了 PI3K 信号中的主要蛋白表达水平，包括 PI3K、Akt、mTOR 和 GSK3。薯蓣皂苷元抑制 OVCAR-3 卵巢癌细胞的增殖和迁移，并诱导凋亡，这可能是通过靶向 PI3K 信号介导的。
