Departments of Radiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.
J Neurosci. 2024 May 8;44(19):e1019232024. doi: 10.1523/JNEUROSCI.1019-23.2024.
A classic example of experience-dependent plasticity is ocular dominance (OD) shift, in which the responsiveness of neurons in the visual cortex is profoundly altered following monocular deprivation (MD). It has been postulated that OD shifts also modify global neural networks, but such effects have never been demonstrated. Here, we use wide-field fluorescence optical imaging (WFOI) to characterize calcium-based resting-state functional connectivity during acute (3 d) MD in female and male mice with genetically encoded calcium indicators (-GCaMP6f). We first establish the fundamental performance of WFOI by computing signal to noise properties throughout our data processing pipeline. Following MD, we found that Δ band (0.4-4 Hz) GCaMP6 activity in the deprived visual cortex decreased, suggesting that excitatory activity in this region was reduced by MD. In addition, interhemispheric visual homotopic functional connectivity decreased following MD, which was accompanied by a reduction in parietal and motor homotopic connectivity. Finally, we observed enhanced internetwork connectivity between the visual and parietal cortex that peaked 2 d after MD. Together, these findings support the hypothesis that early MD induces dynamic reorganization of disparate functional networks including the association cortices.
经典的经验依赖性可塑性的例子是眼优势（OD）移位，在这种情况下，在单眼剥夺（MD）之后，视觉皮层中的神经元的反应性会发生深刻的改变。有人假设 OD 移位也会改变全局神经网络，但这些影响从未得到证明。在这里，我们使用宽场荧光光学成像（WFOI）来描述雌性和雄性携带基因编码钙指示剂（-GCaMP6f）的小鼠在急性（3d）MD 期间基于钙的静息状态功能连接。我们首先通过计算整个数据处理管道中的信噪比特性来确定 WFOI 的基本性能。在 MD 之后，我们发现剥夺视觉皮层中的 Δ 频段（0.4-4Hz）GCaMP6 活性降低，这表明该区域的兴奋性活动因 MD 而减少。此外，MD 后半球间视觉同型功能连接减少，伴随着顶叶和运动同型连接减少。最后，我们观察到视觉皮层和顶叶之间的网络间连接增强，在 MD 后 2d 达到峰值。综上所述，这些发现支持了早期 MD 诱导包括联合皮层在内的不同功能网络的动态重组的假设。
