CDCA5 通过激活 TGF-β1 通路促进人卵巢癌细胞的侵袭和迁移。

CDCA5 promoted cell invasion and migration by activating TGF-β1 pathway in human ovarian cancer cells.

机构信息

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou, 215006, Jiangsu, People's Republic of China.

Department of Gynecological Oncology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, 233004, Anhui, China.

出版信息

J Ovarian Res. 2024 Mar 27;17(1):68. doi: 10.1186/s13048-024-01393-5.

DOI:10.1186/s13048-024-01393-5

PMID:38539247

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10967103/

Abstract

BACKGROUND

The gene cell division cycle associated 5 (CDCA5), also called sororin, has oncogenic characteristics and is upregulated in various carcinomas. Nevertheless, the involvement of CDCA5 in ovarian cancer (OC), a highly aggressive form of cancer, and the underlying mechanism of metastasis remain inadequately investigated.

RESULTS

The bioinformatics data revealed a negative correlation between the patient's survival and CDCA5 expression, which was overexpressed in OC. Functional assays also confirmed high expression levels of CDCA5 in OC tissues and cells. This suggests that CDCA5 may potentially enhance the motility, migration, and proliferation of OC cells invitro. It impedes DNA damage and apoptosis in OC cells, inhibiting xenograft development in nude mice. The RNA sequencing results suggest CDCA5 is majorly associated with biological functions related to the extracellular matrix (ECM) and influences the transforming growth factor (TGF) signaling pathway. Moreover, subsequent functional investigations elucidated that CDCA5 facilitated the migration and invasion of OC cells viathe TGF-β1/Smad2/3 signaling pathway activation.

CONCLUSIONS

CDCA5 may be a strong potential therapeutic target for the treatment and management of OC.

摘要

背景

细胞分裂周期相关蛋白 5（CDCA5）又称为sororin，具有致癌特性，在多种癌症中上调。然而，CDCA5 在卵巢癌（OC）中的作用及其转移的潜在机制仍研究不足，OC 是一种高度侵袭性的癌症。

结果

生物信息学数据显示患者的生存与 CDCA5 表达呈负相关，CDCA5 在 OC 中过表达。功能分析也证实了 CDCA5 在 OC 组织和细胞中的高表达水平。这表明 CDCA5 可能在体外增强 OC 细胞的运动性、迁移性和增殖性。它抑制 OC 细胞中的 DNA 损伤和细胞凋亡，抑制裸鼠异种移植的发展。RNA 测序结果表明 CDCA5 主要与细胞外基质（ECM）相关的生物学功能有关，并影响转化生长因子（TGF）信号通路。此外，随后的功能研究表明 CDCA5 通过 TGF-β1/Smad2/3 信号通路激活促进 OC 细胞的迁移和侵袭。