Tung Yu-Tang, Tung Chun-Liang, Hsieh Cheng-Chia, Huang Yu-Chen, Li Shiming, Tung Chun-Liang, Wu Jyh-Horng
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan.
Advanced Plant and Food Crop Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
Antioxidants (Basel). 2024 Mar 1;13(3):311. doi: 10.3390/antiox13030311.
Soybeans rank among the top five globally produced crops. Black soybeans contain anthocyanins in their seed coat, offering strong antioxidant and anti-inflammatory benefits. This study explores the protective effects of black soybean seed coat (BSSC) against acute liver injury (ALI) in mice. Mice pretreated with BSSC crude extract showed reduced liver damage, inflammation, and apoptosis. High doses (300 mg/kg) of the extract decreased levels of proinflammatory cytokines (IL-6, IFN-γ) and increased levels of anti-inflammatory ones (IL-4, IL-10), alongside mitigating liver pathological damage. Additionally, it influenced the Nrf2/HO-1 pathway and reduced levels of apoptosis-related proteins. In vitro, the compounds delphinidin-3--glucoside (D3G) and cyanidin-3--glucoside (C3G) in BSSC were found to modulate cytokine levels, suggesting their role in ALI protection. The study concludes that BSSC extract, particularly due to D3G and C3G, effectively protects against LPS-induced ALI in mice by inhibiting inflammation, oxidative stress, and apoptosis.
大豆是全球产量最高的五种作物之一。黑豆的种皮中含有花青素，具有强大的抗氧化和抗炎作用。本研究探讨了黑豆种皮（BSSC）对小鼠急性肝损伤（ALI）的保护作用。用BSSC粗提物预处理的小鼠肝脏损伤、炎症和细胞凋亡均有所减轻。高剂量（300 mg/kg）的提取物降低了促炎细胞因子（IL-6、IFN-γ）的水平，提高了抗炎细胞因子（IL-4、IL-10）的水平，同时减轻了肝脏病理损伤。此外，它还影响Nrf2/HO-1通路，降低了凋亡相关蛋白的水平。在体外，发现BSSC中的化合物飞燕草素-3-葡萄糖苷（D3G）和矢车菊素-3-葡萄糖苷（C3G）可调节细胞因子水平，表明它们在ALI保护中发挥作用。该研究得出结论，BSSC提取物，特别是由于D3G和C3G，通过抑制炎症、氧化应激和细胞凋亡，有效保护小鼠免受LPS诱导的ALI。
