Cascajosa-Lira Antonio, Guzmán-Guillén Remedios, Pichardo Silvia, Baños Alberto, de la Torre Jose M, Ayala-Soldado Nahum, Moyano-Salvago M Rosario, Ortiz-Jaraba Isabel, Cameán Ana M, Jos Angeles
Área de Toxicología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
DMC Research Center, Camino de Jayena, 82, 18620 Granada, Spain.
Antioxidants (Basel). 2024 Mar 15;13(3):350. doi: 10.3390/antiox13030350.
Propyl-propane thiosulfonate (PTSO), an antioxidant organosulfur compound present in the genus , has become a potential natural additive for food and feed, as well as a possible biopesticide for pest control in plants. A toxicological assessment is necessary to verify its safety for livestock, consumers, and the environment. As part of the risk assessment of PTSO, this study was designed to explore its potential reproductive toxicity in mice following the OECD 416 guideline. The investigation spans two generations to comprehensively evaluate potential reproductive, teratogenic, and hereditary effects. A total of 80 CD1 mice per sex and generation were subjected to PTSO exposure during three phases (premating, gestation, and lactation). This evaluation encompassed three dose levels: 14, 28, and 55 mg PTSO/kg b.w./day, administered through the feed. No clinical changes or mortality attributed to the administration of PTSO were observed in the study. Some changes in the body weight and food consumption were observed, but not related to sex or in a dose-dependent manner. The two parental generations (F0, F1) exhibited normal reproductive performance, and the offspring (F1 and F2) were born without any abnormalities. The serum sexual hormone levels (progesterone -P-, testosterone -T-, estradiol -E2-, follicular stimulating hormone -FSH-, and luteinizing hormone -LH-) were in a normal range. Although significant changes were observed in the sperm analysis in the case of F0 group, no variation was found for F1 group, and no alterations in fertility were recorded either. The absolute organ weights and relative organ weight/body weight and organ weight/brain weight ratios, and the complete histopathological study, showed no significant alterations in males and females for all the generations considered. Considering all the results obtained, PTSO is not considered a reproductive or developmental toxicant in mice under the assayed conditions. These results support the good safety profile of PTSO for its potential application in the agrifood sector.
丙基丙烷硫代磺酸盐（PTSO）是一种存在于该属中的抗氧化有机硫化合物，已成为食品和饲料的潜在天然添加剂，也是植物害虫防治的一种可能的生物农药。进行毒理学评估以验证其对牲畜、消费者和环境的安全性。作为PTSO风险评估的一部分，本研究旨在按照经合组织416指南探索其对小鼠的潜在生殖毒性。该调查涵盖两代，以全面评估潜在的生殖、致畸和遗传效应。每个性别和世代共有80只CD1小鼠在三个阶段（交配前、妊娠期和哺乳期）接受PTSO暴露。该评估包括三个剂量水平：14、28和55毫克PTSO/千克体重/天，通过饲料给药。在研究中未观察到因给予PTSO而导致的临床变化或死亡。观察到体重和食物摄入量有一些变化，但与性别无关，也不是剂量依赖性的。两代亲本（F0、F1）表现出正常的生殖性能，后代（F1和F2）出生时没有任何异常。血清性激素水平（孕酮 -P-、睾酮 -T-、雌二醇 -E2-、促卵泡激素 -FSH- 和促黄体生成素 -LH-）在正常范围内。虽然在F0组的精子分析中观察到显著变化，但F1组未发现差异，生育力也未记录到改变。所有考虑世代的雄性和雌性的绝对器官重量、相对器官重量/体重和器官重量/脑重量比值以及完整的组织病理学研究均未显示出显著改变。考虑到所有获得的结果，在测定条件下，PTSO不被认为是小鼠的生殖或发育毒物。这些结果支持了PTSO在农业食品领域潜在应用中的良好安全性。
