National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Genes (Basel). 2024 Feb 27;15(3):307. doi: 10.3390/genes15030307.
As a plant-specific transcription factor, the vascular plant one-zinc-finger (VOZ) plays a crucial role in regulating various biological processes. In this study, a total of 17 genes in the Cucurbitaceae family were investigated using various bioinformatics methods. The 17 genes in Cucurbitaceae are distributed across 16 chromosomes. Based on the affinity of VOZ proteins to AtVOZ proteins, these 17 proteins were categorized into two groups: group I encompassed eight VOZ members, while group II comprised nine VOZ members. The expression profiles of CmoVOZs under various hormonal and abiotic stresses indicated that these genes were induced differentially by JA, ABA, GA, salt, and drought stress. Subsequently, CmoVOZ1 and CmoVOZ2 were found to be transcriptionally active, with the CmoVOZ2 protein being located mainly in the nucleus. Further experiments revealed that yeast cells expressing gene showed increased tolerance to salt stress and drought stress. These results suggest that the gene family is not only important for plant growth and development but also that this mechanism may be universal across yeast and plants.
作为一种植物特异性转录因子，维管束植物单锌指（VOZ）在调节各种生物过程中起着至关重要的作用。在这项研究中，使用各种生物信息学方法研究了葫芦科中的 17 个基因。葫芦科中的 17 个基因分布在 16 条染色体上。根据 VOZ 蛋白与 AtVOZ 蛋白的亲和力，这些 17 个蛋白被分为两组：第一组包括 8 个 VOZ 成员，第二组包括 9 个 VOZ 成员。CmoVOZs 在各种激素和非生物胁迫下的表达谱表明，这些基因被 JA、ABA、GA、盐和干旱胁迫不同程度地诱导。随后发现 CmoVOZ1 和 CmoVOZ2 具有转录活性，CmoVOZ2 蛋白主要位于细胞核中。进一步的实验表明，表达基因的酵母细胞对盐胁迫和干旱胁迫的耐受性增加。这些结果表明，该基因家族不仅对植物的生长和发育很重要，而且这种机制可能在酵母和植物中是普遍存在的。
