Du Juan, Ni Zhi-Jing, Wang Wei, Thakur Kiran, Ma Run-Hui, Ma Wen-Ping, Wei Zhao-Jun
School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China.
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Foods. 2024 Mar 21;13(6):955. doi: 10.3390/foods13060955.
Carbon dots (CDs) have been proposed as photosensitizers in photodynamic treatment (PDT), owing to their excellent biological attributes and budding fruit preservation applications. In the present study, CDs (4.66 nm) were synthesized for photodynamic treatment to improve the quality attributes in post-harvest goji berries. The prepared CDs extended the storage time of the post-harvest goji berries by 9 d. The CD-mediated PDT postponed the hardness and decay index loss, reduced the formation of malondialdehyde (MDA), hydrogen peroxide (HO), and superoxide anion (O) significantly, and delayed the loss of vital nutrients like the total protein, phenols, and flavonoids. The CD-mediated PDT improved the catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD), phenylalanine ammonia-lyase (PAL), glutathione reductase (GR), and superoxide dismutase (SOD) activities, but did not improve polyphenol oxidase (PPO) activity. In addition, The CD-mediated PDT induced the accumulation of ascorbic acid (ASA) and glutathione (GSH). Overall, a CD-mediated PDT could extend the storage time and augment the quality attributes in post-harvest fresh goji berries by regulating the antioxidant system.
由于碳点（CDs）具有出色的生物学特性以及在水果保鲜方面的潜在应用，它们已被提议用作光动力疗法（PDT）中的光敏剂。在本研究中，合成了尺寸为4.66纳米的碳点用于光动力处理，以改善采后枸杞的品质特性。制备的碳点使采后枸杞的储存时间延长了9天。碳点介导的光动力疗法延缓了硬度和腐烂指数的下降，显著减少了丙二醛（MDA）、过氧化氢（HO）和超氧阴离子（O）的生成，并延缓了总蛋白、酚类和黄酮类等重要营养成分的损失。碳点介导的光动力疗法提高了过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）、过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）、谷胱甘肽还原酶（GR）和超氧化物歧化酶（SOD）的活性，但未提高多酚氧化酶（PPO）的活性。此外，碳点介导的光动力疗法诱导了抗坏血酸（ASA）和谷胱甘肽（GSH）的积累。总体而言，碳点介导的光动力疗法可通过调节抗氧化系统来延长采后新鲜枸杞的储存时间并增强其品质特性。
