采用数字 PCR 对子宫内膜癌中选定 microRNAs 表达的绝对定量。

Absolute Quantification of Selected microRNAs Expression in Endometrial Cancer by Digital PCR.

机构信息

Department of Gynecology, Gynecology Oncology and Obstetrics, Fryderyk Chopin University Hospital, 35-055 Rzeszow, Poland.

Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, Medical College of Rzeszow University, 35-959 Rzeszow, Poland.

出版信息

Int J Mol Sci. 2024 Mar 14;25(6):3286. doi: 10.3390/ijms25063286.

DOI:10.3390/ijms25063286

PMID:38542261

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10969921/

Abstract

MicroRNAs (miRNA) are involved in the process of carcinogenesis, including the development of endometrial cancer (EC). This study aimed to investigate the association between the expression of three miRNAs (miR-21-5p, miR-205-5p, and miR-222-3p) in endometrial cancer tissues. In addition, the stability of expression of SNORD48 and U6, which were initially planned to be used as reference miRNAs for normalization, was investigated. Endometrial tissue was obtained from 111 patients with EC during hysterectomy and from 19 patients undergoing surgery for uterine fibroids or pelvic organ prolapse as a control group without neoplastic changes. Our study was based on calculations made with a digital PCR method (Qiagen, Hilden, Germany) to measure the absolute expression. In the endometrial cancer tissue, miR-205-5p was upregulated, while miR-222-3p and SNORD48 were downregulated compared to the control group. We detected statistically significant correlation of miR-205-5p, U6, and SNORD48 expression with different histological grades; the expression of miR-205-5p increases with the histopathological grade advancement (intraepithelial neoplasia- EIN = 1590, G1 = 3367.2, G2 = 8067 and G3 = 20,360), while U6 and SNORD expression decreases from EIN to G2 and increases again in the G3 grade (U6: EIN = 19,032, G1 = 16,482.4, G2 = 13,642.4, G3 = 133,008; SNORD48: EIN = 97,088, G1 = 59,520, G2 = 43,544, G3 = 227,200). Our study suggests that upregulation of miR-205-5p and downregulation of miR-222-3p and SNORD48 may influence development of endometrial cancer. Moreover, miR-205-5p, U6, and SNORD48 expression changes may be associated with progression of endometrial cancer. The results also indicate that SNORD48 and U6, commonly used as internal references, may influence endometrial cancer development and progression; therefore, they should not be used as references. However, it is important to note that further research is required to understand their role in endometrial cancer.

摘要

微小 RNA（miRNA）参与了致癌过程，包括子宫内膜癌（EC）的发展。本研究旨在探讨三种 miRNA（miR-21-5p、miR-205-5p 和 miR-222-3p）在子宫内膜癌组织中的表达与子宫内膜癌的关系。此外，还研究了最初计划用作归一化内参的 SNORD48 和 U6 的表达稳定性。从 111 例因子宫切除术而患有 EC 的患者和 19 例因子宫肌瘤或盆腔器官脱垂而行手术的患者（无肿瘤性改变的对照组）中获得子宫内膜组织。我们的研究基于数字 PCR 方法（德国 Hilden 的 Qiagen）进行绝对表达计算。在子宫内膜癌组织中，miR-205-5p 上调，而 miR-222-3p 和 SNORD48 下调与对照组相比。我们检测到 miR-205-5p、U6 和 SNORD48 表达与不同组织学分级之间存在统计学显著相关性；miR-205-5p 的表达随着组织病理学分级的进展而增加（上皮内瘤变-EIN=1590、G1=3367.2、G2=8067 和 G3=20，360），而 U6 和 SNORD 的表达则从 EIN 到 G2 降低，然后在 G3 级再次升高（U6：EIN=19，032、G1=16，482.4、G2=13，642.4、G3=133，008；SNORD48：EIN=97，088、G1=59，520、G2=43，544、G3=227，200）。我们的研究表明，miR-205-5p 的上调和 miR-222-3p 和 SNORD48 的下调可能影响子宫内膜癌的发展。此外，miR-205-5p、U6 和 SNORD48 表达的变化可能与子宫内膜癌的进展有关。结果还表明，通常用作内参的 SNORD48 和 U6 可能会影响子宫内膜癌的发生和发展；因此，它们不应作为参考。但是，需要注意的是，需要进一步研究才能了解它们在子宫内膜癌中的作用。