Department of Obstetrics and Gynecology, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, 750002 Ningxia, China.
Comput Math Methods Med. 2022 Jul 20;2022:9304392. doi: 10.1155/2022/9304392. eCollection 2022.
Long noncoding RNA neuroblastoma-associated transcript 1 (NBAT1) is implicated in the progression of various cancers. Nevertheless, its biological function in endometrial cancer (EC) remains unknown.
The levels of NBAT1, miR-21-5p, and PTEN in EC cells and EC tissues were examined by RT-qPCR. Western blot was carried out to assess the protein expression of PTEN. The dual-luciferase reporter assay was conducted to explore the interactions among NBAT1, miR-21-5p, and PTEN. The effect of NBAT1 on EC proliferation, metastasis, and apoptosis was evaluated by CCK-8, transwell assays, wound healing, and flow cytometry. miR-21-5p mimics or NBAT1+miR-21-5p were transfected into HEC-1A and Ishikawa cells to investigate whether NBAT1 regulated EC tumorigenesis via sponging miR-21-5p.
NBAT1 is downregulated, and miR-21-5p is upregulated in EC cells and tumor tissues. Overexpression of NBAT1 inhibits the proliferation, migration, and invasion abilities of EC cells and facilitated apoptosis. NBAT1 directly binds and negatively regulates miR-21-5p in EC. miR-21-5p mimics reverses the effect of lncRNA NBAT1 overexpression on the proliferation and migration of EC cells. PTEN is a downstream gene of miR-21-5p. lncRNA NBTA1 elevates PTEN expression via sponging miR-21-5p.
lncRNA NBAT1 acts as a tumor suppressor in EC via regulating PTEN through sponging miR-21-5p.
长链非编码 RNA 神经母细胞瘤相关转录物 1（NBAT1）参与多种癌症的进展。然而，其在子宫内膜癌（EC）中的生物学功能尚不清楚。
通过 RT-qPCR 检测 EC 细胞和组织中 NBAT1、miR-21-5p 和 PTEN 的水平。Western blot 用于评估 PTEN 的蛋白表达。双荧光素酶报告基因实验探讨 NBAT1、miR-21-5p 和 PTEN 之间的相互作用。通过 CCK-8、Transwell 实验、划痕愈合和流式细胞术评估 NBAT1 对 EC 增殖、转移和凋亡的影响。将 miR-21-5p 模拟物或 NBAT1+miR-21-5p 转染到 HEC-1A 和 Ishikawa 细胞中，以研究 NBAT1 是否通过海绵 miR-21-5p 调节 EC 肿瘤发生。
NBAT1 在 EC 细胞和肿瘤组织中下调，miR-21-5p 上调。NBAT1 的过表达抑制 EC 细胞的增殖、迁移和侵袭能力，并促进凋亡。NBAT1 在 EC 中直接结合并负调控 miR-21-5p。miR-21-5p 模拟物逆转 lncRNA NBAT1 过表达对 EC 细胞增殖和迁移的影响。PTEN 是 miR-21-5p 的下游基因。lncRNA NBTA1 通过海绵 miR-21-5p 上调 PTEN 表达。
lncRNA NBAT1 通过海绵 miR-21-5p 调节 PTEN，在 EC 中作为肿瘤抑制因子发挥作用。
