Department of Pathology, Avera Cancer Institute, Sioux Falls, SD 57108, USA.
Translational Oncology Laboratory, Avera Cancer Institute, Sioux Falls, SD 57108, USA.
Int J Mol Sci. 2024 Mar 19;25(6):3441. doi: 10.3390/ijms25063441.
Lymphovascular invasion (LVSI) is defined as the presence of tumor cells within a definite endothelial-lined space (lymphatics or blood vessels) in the organ surrounding invasive carcinoma. The presence of LVI is associated with an increased risk of lymph nodes and distant metastases. Lymphovascular invasion is described as cancer within blood or lymph vessels and is an independent risk factor for metastasis, recurrence, and mortality. This study aims to present the marker-based immunohistological characterization of cells around LVSI in a high-grade adenocarcinoma of the endometrium to build a cellular atlas of cells of LVSI. A cellular characterization of the cells around lymphovascular space invasion in a 67-year-old female patient with invasive high-grade serous endometrial adenocarcinomas is presented. Resected tumor tissue from a consented patient with invasive high-grade serous endometrial adenocarcinoma was obtained within an hour of surgery. The expressions of the epithelial markers (CK8, 18, and EpCAM), LCA (leukocyte common antigen) marker (CD45), proliferation marker (Ki67), apoptosis markers (cleaved PARP and cleaved caspase3), immune cell markers (CD3, CD4, CD8, CD56, CD68, CD163, FoxP3, PD-1, PD-L1), pro-inflammatory marker (IL-12-RB2), and fibroblast/mesenchyme markers (S100A7, SMA, and TE-7) of the resected tissue on the IHC stains were evaluated and scored by a pathologist. Acknowledging the deterministic role of LVSI in a high-grade adenocarcinoma of the endometrium, our study presents the first marker-based immunohistological atlas of the tumor and TME compartments in the context of epithelial cell markers, proliferation markers, apoptosis markers, macrophage markers, and fibroblast markers. Our study demonstrates that an aggressive disease like a high-grade adenocarcinoma of the endometrium inflicts the pro-metastatic event of LVSI by involving the immune landscape of both tumor and TME. This study demonstrates, for the first time, that the tumor cells within LVSI are positive for IL-12R-B2 and S100A4.
脉管侵犯（LVSI）定义为在周围浸润性癌器官中，肿瘤细胞位于明确的内皮衬里空间（淋巴管或血管）内。LVI 的存在与淋巴结和远处转移的风险增加有关。脉管侵犯被描述为血液或淋巴管内的癌症，是转移、复发和死亡的独立危险因素。本研究旨在展示高级别子宫内膜腺癌中 LVSI 周围基于标志物的免疫组织化学特征，以构建 LVSI 细胞的细胞图谱。展示了一位 67 岁女性患者浸润性高级别浆液性子宫内膜腺癌中淋巴管侵犯周围细胞的细胞特征。在手术后一小时内从一位经同意的患有浸润性高级别浆液性子宫内膜腺癌的患者中获得切除的肿瘤组织。对切除组织进行了免疫组化染色，评估并评分了上皮标志物（CK8、18 和 EpCAM）、LCA（白细胞共同抗原）标志物（CD45）、增殖标志物（Ki67）、凋亡标志物（cleaved PARP 和 cleaved caspase3）、免疫细胞标志物（CD3、CD4、CD8、CD56、CD68、CD163、FoxP3、PD-1、PD-L1）、促炎标志物（IL-12-RB2）和纤维母细胞/间充质标志物（S100A7、SMA 和 TE-7）的表达。本研究承认 LVSI 在高级别子宫内膜腺癌中的决定性作用，首次展示了上皮细胞标志物、增殖标志物、凋亡标志物、巨噬细胞标志物和纤维母细胞标志物背景下肿瘤和 TME 区室的基于标志物的免疫组织学图谱。我们的研究表明，像高级别子宫内膜腺癌这样的侵袭性疾病通过涉及肿瘤和 TME 的免疫景观，引发 LVSI 的促转移事件。本研究首次证明，LVSI 内的肿瘤细胞呈 IL-12R-B2 和 S100A4 阳性。
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