Khubiev Omar M, Egorov Anton R, Semenkova Daria I, Salokho Darina S, Golubev Roman A, Sikaona Nkumbu D, Lobanov Nikolai N, Kritchenkov Ilya S, Tskhovrebov Alexander G, Kirichuk Anatoly A, Khrustalev Victor N, Kritchenkov Andreii S
Department of Human Ecology and Bioelementology, RUDN University, Miklukho-Maklaya St. 6, Moscow 117198, Russia.
Metal Physics Laboratory, Institute of Technical Acoustics NAS of Belarus, General Lyudnikov Ave. 13, 210009 Vitebsk, Belarus.
Polymers (Basel). 2024 Mar 9;16(6):755. doi: 10.3390/polym16060755.
In this study, Rhodamine B-containing chitosan-based films were prepared and characterized using their mechanical, photophysical, and antibacterial properties. The films were synthesized using the casting method and their mechanical properties, such as tensile strength and elongation at break, were found to be dependent on the chemical composition and drying process. Infrared spectroscopy and X-ray diffraction analysis were used to examine the chemical structure and degree of structural perfection of the films. The photophysical properties of the films, including absorption spectra, fluorescence detection, emission quantum yields, and lifetimes of excited states, were studied in detail. Rhodamine B-containing films exhibited higher temperature sensitivity and showed potential as fluorescent temperature sensors in the physiological range. The antibacterial activity of the films was tested against Gram-positive bacteria and Gram-negative bacteria , with Rhodamine B-containing films demonstrating more pronounced antibacterial activity compared to blank films. The findings suggest that the elaborated chitosan-based films, particularly those containing Rhodamine B can be of interest for further research regarding their application in various fields such as clinical practice, the food industry, and agriculture due to their mechanical, photophysical, and antibacterial properties.
在本研究中,制备了含罗丹明B的壳聚糖基薄膜,并利用其机械性能、光物理性能和抗菌性能对其进行了表征。采用流延法合成了薄膜,发现其机械性能,如拉伸强度和断裂伸长率,取决于化学成分和干燥过程。利用红外光谱和X射线衍射分析来研究薄膜的化学结构和结构完善程度。详细研究了薄膜的光物理性能,包括吸收光谱、荧光检测、发射量子产率和激发态寿命。含罗丹明B的薄膜表现出更高的温度敏感性,并在生理范围内显示出作为荧光温度传感器的潜力。测试了薄膜对革兰氏阳性菌和革兰氏阴性菌的抗菌活性,与空白薄膜相比,含罗丹明B的薄膜表现出更显著的抗菌活性。研究结果表明,所制备的壳聚糖基薄膜,特别是那些含有罗丹明B的薄膜,由于其机械性能、光物理性能和抗菌性能,在临床实践、食品工业和农业等各个领域的应用方面可能值得进一步研究。