Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China.
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China.
Sensors (Basel). 2024 Mar 7;24(6):1740. doi: 10.3390/s24061740.
Ofloxacin (OFL) is widely used in animal husbandry and aquaculture due to its low price and broad spectrum of bacterial inhibition, etc. However, it is difficult to degrade and is retained in animal-derived food products, which are hazardous to human health. In this study, a simple and efficient method was developed for the detection of OFL residues in meat products. OFL coupled with amino magnetic beads by an amination reaction was used as a stationary phase. Aptamer AWO-06, which showed high affinity and specificity for OFL, was screened using the exponential enrichment (SELEX) technique. A fluorescent biosensor was developed by using AWO-06 as a probe and graphene oxide (GO) as a quencher. The OFL detection results could be obtained within 6 min. The linear range was observed in the range of 10-300 nM of the OFL concentration, and the limit of the detection of the sensor was 0.61 nM. Furthermore, the biosensor was stored at room temperature for more than 2 months, and its performance did not change. The developed biosensor in this study is easy to operate and rapid in response, and it is suitable for on-site detection. This study provided a novel method for the detection of OFL residues in meat products.
氧氟沙星（OFL）由于其价格低廉、抑菌谱广等特点，在畜牧业和水产养殖业中得到了广泛应用。然而，它难以降解，并残留在动物源性食品中，对人类健康构成危害。在本研究中，开发了一种简单高效的方法来检测肉类产品中的 OFL 残留。通过氨化反应将 OFL 与氨基磁性珠偶联，作为固定相。采用指数富集（SELEX）技术筛选出对 OFL 具有高亲和力和特异性的适配体 AWO-06。利用 AWO-06 作为探针，氧化石墨烯（GO）作为猝灭剂，开发了一种荧光生物传感器。该传感器在 OFL 浓度为 10-300 nM 的范围内呈现出线性范围，检测限为 0.61 nM。此外，该传感器在室温下储存超过 2 个月，其性能没有变化。本研究开发的生物传感器操作简单，响应迅速，适用于现场检测。本研究为检测肉类产品中的 OFL 残留提供了一种新方法。
