Sega G A
Genetics. 1979 May;92(1 Pt 1 Suppl):s49-58.
Unscheduled DNA synthesis (UDS) has been found to occur in vivo in specific meiotic and postmeiotic germ cell stages of male mice after mutagen treatment. Techniques and problems involved in measuring UDS are discussed, including the administration of tritium-labeled thymidine ([3H]dT) and mutagens, and the detection of UDS using liquid scintillation counting (LSC) and autoradiography. The sensitivity of detection of UDS in the male germ cells is compared to that of other genetic end points, and the possible role of UDS in the actual repair of genetic damage is considered. Possible areas for future research on the role of UDS in repair of genetic damage in the mouse are suggested.
已发现，在诱变处理后，雄性小鼠特定的减数分裂期和减数分裂后生殖细胞阶段会在体内发生非程序性DNA合成（UDS）。本文讨论了测量UDS所涉及的技术和问题，包括氚标记胸腺嘧啶核苷（[3H]dT）和诱变剂的施用，以及使用液体闪烁计数（LSC）和放射自显影检测UDS。将雄性生殖细胞中UDS的检测灵敏度与其他遗传终点的检测灵敏度进行了比较，并探讨了UDS在实际修复遗传损伤中的可能作用。还提出了未来关于UDS在小鼠遗传损伤修复中作用的研究可能领域。
