Sega G A
Basic Life Sci. 1980;15:373-87. doi: 10.1007/978-1-4684-3842-0_26.
Unscheduled DNA synthesis (UDS) induced in the germ cells of male mice by chemical and physical agents can be studied in vivo by making use of the timing of spermatogenesis and spermiogenesis. In meiotic and postmeiotic germ-cell stages, UDS occurs from leptotene through midspermatid stages but is not detected in later stages. No consistent correlation has been seen between the occurrence of UDS in the germ cells and reduced dominant lethal frequencies or reduced specific-locus mutation frequencies. It is suggested that the UDS observed in the germ cells may be principally involved in the removal of DNA lesions which, if left, could give rise to subtle genetic damage that current mammalian genetic tests may not be able to detect. Characterization of mouse stocks with reduced UDS capability in their germ cells plus the development of biochemical genetic markers that can measure single amino acid substitutions will likely be necessary before the relationship between UDS in mammalian germ cells and repair of genetic damage can be clearly established.
利用精子发生和精子形成的时间，可以在体内研究化学和物理因子诱导雄性小鼠生殖细胞中的非程序性DNA合成（UDS）。在减数分裂和减数分裂后生殖细胞阶段，UDS发生于细线期至精子细胞中期，但在后期未检测到。生殖细胞中UDS的发生与显性致死频率降低或特定基因座突变频率降低之间未发现一致的相关性。有人提出，在生殖细胞中观察到的UDS可能主要参与DNA损伤的修复，这些损伤若不修复，可能会导致目前哺乳动物遗传检测无法检测到的细微遗传损伤。在能够明确建立哺乳动物生殖细胞中UDS与遗传损伤修复之间的关系之前，可能有必要对生殖细胞中UDS能力降低的小鼠品系进行表征，并开发能够检测单个氨基酸替代的生化遗传标记。
