Kim Jinman, Lee Yejin, Suh Hee-Jae, Lee Hee-Seok, Lee Chan
Department of Food Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do, Republic of Korea.
Department of Food Science, Sun Moon University, Asan, Chungchengnam-do, Republic of Korea.
Biosci Biotechnol Biochem. 2024 May 22;88(6):639-647. doi: 10.1093/bbb/zbae037.
Efficient extraction of natural pigments is a key focus in enhancing the utilization of by-products for applications in the food industry. In this study, an enzymatic extraction method using Pectinex Ultra SP-L, Pectinex XXL, Novoshape, and Celluclast was used to investigate natural pigment production from the pomace of aronia, a commercially important plant. The method's performance was monitored using high-performance liquid chromatography with diode-array detection by measuring total and individual anthocyanin levels. Pectinex XXL (0.5%) yielded the highest total anthocyanin extraction (2082.41 ± 85.69 mg/100 g) in the single enzyme treatment, followed by Pectinex Ultra SP-L (0.05%), Celluclast (0.01%), and Novoshape (0.1%). Combining Pectinex XXL (0.25%) with Celluclast (0.01%) increased the extraction ratio of total anthocyanins (2 323.04 ± 61.32 mg/100 g) by ∼50.7% compared with that obtained using the solvent extraction method. This study demonstrated an effective enzymatic extraction method for application in the food industry.
高效提取天然色素是提高食品工业副产品利用率的关键重点。在本研究中，使用果胶酶Ultra SP-L、果胶酶XXL、诺维信酶制剂和纤维素酶的酶法提取方法来研究从商业上重要的植物黑果腺肋花楸果渣中生产天然色素。通过使用二极管阵列检测的高效液相色谱法测量总花青素和单个花青素水平来监测该方法的性能。在单酶处理中，果胶酶XXL（0.5%）的总花青素提取量最高（2082.41±85.69毫克/100克），其次是果胶酶Ultra SP-L（0.05%）、纤维素酶（0.01%）和诺维信酶制剂（0.1%）。与溶剂提取法相比，将果胶酶XXL（0.25%）与纤维素酶（0.01%）相结合可使总花青素的提取率提高约50.7%（2323.04±61.32毫克/100克）。本研究证明了一种适用于食品工业的有效酶法提取方法。
