A Sujitha, Girija A S Smiline, Priyadharsini Vijayashree J
Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND.
Department of Clinical Genetics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND.
Cureus. 2024 Feb 26;16(2):e54909. doi: 10.7759/cureus.54909. eCollection 2024 Feb.
The gene in is attributed for its virulence by producing the enzyme serine hydroxymethyltransferase (SHMT), which plays a vital role in bacterial cell metabolism.
The study is thus aimed to determine the frequency of the gene from the clinical strains of isolated from periodontitis patients.
Forty-five patients with varying degrees of periodontitis were included in the study, and the plaque samples collected from them were anaerobically processed by inoculating onto sterile anaerobic blood agar plates using a gaspak system, with incubation at 37°C for 5-7 days. The DNA was extracted from the obtained isolated colony, and PCR was performed to confirm the presence of the gene.
In total, 46.6% (= 7) of the cases in group III aggressive periodontitis and 6.66% (1) in group II stage II periodontitis showed the presence of , and among them, 57.14% (= 4) showed the presence of the gene. Conclusion: The findings of the study showed that the gene may be associated with the pathogenesis of and could be thus a novel candidate for the future theragnostic approach to combat periodontitis.
中的基因通过产生丝氨酸羟甲基转移酶（SHMT）发挥其毒力作用，该酶在细菌细胞代谢中起关键作用。
本研究旨在确定从牙周炎患者分离出的临床菌株中的基因频率。
本研究纳入45例不同程度牙周炎患者，采集其菌斑样本，使用气体Pak系统接种于无菌厌氧血琼脂平板上进行厌氧处理，于37°C孵育5 - 7天。从获得的分离菌落中提取DNA，并进行PCR以确认基因的存在。
在III组侵袭性牙周炎病例中，共有46.6%（= 7），在II组II期牙周炎病例中6.66%（1）显示存在，其中57.14%（= 4）显示存在基因。结论：研究结果表明，基因可能与的发病机制有关，因此可能成为未来对抗牙周炎的治疗诊断方法的新候选靶点。
