Molecular Characterization of the glyA Gene From the Clinical Isolates of Tannerella forsythia.

BACKGROUND

The  gene in is attributed for its virulence by producing the enzyme serine hydroxymethyltransferase (SHMT), which plays a vital role in bacterial cell metabolism.

OBJECTIVES

The study is thus aimed to determine the frequency of the gene from the clinical strains of isolated from periodontitis patients.

MATERIALS AND METHODS

Forty-five patients with varying degrees of periodontitis were included in the study, and the plaque samples collected from them were anaerobically processed by inoculating onto sterile anaerobic blood agar plates using a gaspak system, with incubation at 37°C for 5-7 days. The DNA was extracted from the obtained isolated colony, and PCR was performed to confirm the presence of the gene.

RESULTS

In total, 46.6% (= 7) of the cases in group III aggressive periodontitis and 6.66% (1) in group II stage II periodontitis showed the presence of , and among them, 57.14% (= 4) showed the presence of the  gene.  Conclusion: The findings of the study showed that the  gene may be associated with the pathogenesis of and could be thus a novel candidate for the future theragnostic approach to combat periodontitis.