Analysis of the Expression of in Patients with Hepatocellular Carcinoma and its Effect on the Phenotype of Hepatocellular Carcinoma Cells.

OBJECTIVES

This research aimed to study the expression of mRNA in hepatocellular carcinoma (HCC) and its effect on the prognosis of HCC. Moreover, the effect of gene knockdown on the proliferation, migration, and invasion of HepG2 cells mediated by lentivirus was also examined. This study offers a theoretical and experimental basis for further research on the mechanism of PRDX6 in liver cancer and new methods for clinical diagnosis and treatment.

METHODS

RNA sequence data of 369 HCC patients were screened through the TCGA database, and the expression and clinical characteristics of mRNA were analyzed based on high-throughput RNA sequencing data. HepG2 cells were divided into WT, sh-NC and sh- groups. Real-time PCR and Western blot were used to detect the expression levels of the gene and protein, respectively. CCK8 method was used to detect the proliferation activity of HepG2 cells, scratch healing test was used to detect the migration ability, Transwell chamber was used to detect the invasion ability, and Western blot was used to detect the expression levels of PI3K/Akt/mTOR signaling pathway and Notch signaling pathway-related proteins.

RESULTS

The expression of was significantly correlated with the gender, race, clinical stage, histological grade, and survival time of HCC patients ( < 0.05). Compared with that in WT and sh-NC groups, the expression level of protein in HCC patients was significantly lower ( < 0.01), the proliferation activity of HCC cells was significantly decreased ( < 0.05), and the migration and invasion ability was significantly decreased ( < 0.05) in the sh- group. The expression levels of PI3K, p-Akt, p-mTOR, Notch1, and Hes1 proteins in the sh- group were significantly lower than those in WT and sh-NC groups ( < 0.05).

CONCLUSION

The expression of may be closely related to the prognosis of HCC. Lentivirus-mediated knockdown can inhibit the proliferation, migration and invasion of HCC cells, which may be related to its regulating the PI3K/Akt/mTOR and Notch1 signaling pathways. is expected to be a new target for the diagnosis and treatment of liver cancer.