Wei Peiying, Hu Qiuhui, He Chengbin, Hua Peng, Yang Di, Shao Chang, Xie Lesi, Han Zhijiang, Zhou Xiaoxuan, Ding Zhongxiang, Hu Hongjie
Department of Radiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Department of Radiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Heliyon. 2024 Mar 8;10(6):e27419. doi: 10.1016/j.heliyon.2024.e27419. eCollection 2024 Mar 30.
To investigate gadolinium deposition in the liver and brain in a rat model with liver fibrosis (LF) after intravenous administration of gadoxetate disodium (GD) and the histological effects of gadolinium deposition in the liver and brain.
Adult male Sprague-Dawley rats were randomly assigned to one of the three groups: 1) LF group received intraperitoneal injection of carbon tetrachloride (CCl) for 9 weeks alone; 2) LF&GD group received CCl and intravenous administration of GD (for 5 consecutive days); 3) GD group received olive oil and GD. Seven days after the final injection of GD, the deep cerebellar nuclei (DCN) and liver were excised to determine gadolinium concentrations via inductively coupled plasma mass spectrometry, and histologic staining was performed. Bonferroni's post-hoc test and Wilcoxon rank sum test were used to compare the differences between the three groups.
The concentrations of retained gadolinium in the liver in the LF&GD group (2.18 ± 0.44 μg/g) were significantly greater compared to the LF group (0.02 ± 0.01 μg/g, < 0.001) and GD group (0.37 ± 0.11 μg/g, < 0.001). Also, the concentrations of retained gadolinium in DCN were increased in the LF&GD group (0.13 ± 0.06 μg/g) compared to the LF group (0.01 ± 0.00 μg/g, < 0.001) and GD group (0.06 ± 0.02 μg/g, = 0.019). No histopathological alterations were detected in the liver and DCN between LF&GD group and LF group.
LF aggravated gadolinium deposition in the liver and DCN after administration of GD. However, no significant acute histological alterations were observed due to gadolinium deposition.
研究静脉注射钆塞酸二钠（GD）后肝纤维化（LF）大鼠模型肝脏和脑内钆的沉积情况以及钆沉积对肝脏和脑的组织学影响。
成年雄性Sprague-Dawley大鼠随机分为三组：1）LF组，仅腹腔注射四氯化碳（CCl）9周；2）LF&GD组，腹腔注射CCl并静脉注射GD（连续5天）；3）GD组，给予橄榄油和GD。最后一次注射GD 7天后，切除小脑深部核团（DCN）和肝脏，通过电感耦合等离子体质谱法测定钆浓度，并进行组织学染色。采用Bonferroni事后检验和Wilcoxon秩和检验比较三组间差异。
LF&GD组肝脏中钆的留存浓度（2.18±0.44μg/g）显著高于LF组（0.02±0.01μg/g，<0.001）和GD组（0.37±0.11μg/g，<0.001）。此外，LF&GD组DCN中钆留存浓度（0.13±0.06μg/g）高于LF组（0.01±0.00μg/g，<0.001）和GD组（0.06±0.02μg/g，=0.019）。LF&GD组与LF组肝脏和DCN均未检测到组织病理学改变。
LF加重了GD给药后肝脏和DCN中钆的沉积。然而，未观察到钆沉积引起明显的急性组织学改变。
