铁皮石斛内生菌代谢产物通过Nrf2/Keap1途径对SH-SY5Y细胞的神经保护作用
Neuroprotective effects of dendrobium endophytes metabolites in SH-SY5Y cells via the Nrf2/Keap1 pathway.
作者信息
Liang Yan Tian, Liu Jia Meng, Qin Lu Qi, Lu Cong, Sun Jing, Wang Qiong, Yang Yong, Fan Bei, Wang Feng Zhong
机构信息
College of Pharmacy, Hunan University of Traditional Chinese Medicine, Hunan, China.
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
出版信息
Heliyon. 2024 Mar 18;10(6):e28222. doi: 10.1016/j.heliyon.2024.e28222. eCollection 2024 Mar 30.
Recent studies have revealed that endophytes in plants can produce metabolites with activity that is comparable to or identical to the host. Dendrobine has attracted much attention in the field of neurodegenerative diseases by exhibiting anti-oxidative stress and neuroprotective effects. This study aimed to investigate the protective effects and mechanisms of metabolites of dendrobium endophytes CM-YJ44 and D-HT207 against HO-induced oxidative stress injury in SH-SY5Y cells. Results showed that there were 50 neuroprotective compounds in CM-YJ44 and 72 neuroprotective compounds in D-HT207. Those both increased significantly cell viability, decreased contents of ROS in HO-induced SH-SY5Y cells. It was confirmed that metabolites of CM-YJ44 and D-HT207 inhibited the HO-induced oxidative stress injury in SH-SY5Y cells, which mechanism is related to inhibition of ROS production, alteration of MMP, and inhibition of apoptosis and inflammatory factors expression via the Nrf2/Keap1 pathway.
最近的研究表明，植物内生菌可以产生与宿主具有相当或相同活性的代谢产物。石斛碱通过表现出抗氧化应激和神经保护作用，在神经退行性疾病领域引起了广泛关注。本研究旨在探讨石斛内生菌CM-YJ44和D-HT207的代谢产物对HO诱导的SH-SY5Y细胞氧化应激损伤的保护作用及其机制。结果显示，CM-YJ44中有50种神经保护化合物，D-HT207中有72种神经保护化合物。两者均显著提高了HO诱导的SH-SY5Y细胞的活力，降低了细胞内活性氧含量。证实CM-YJ44和D-HT207的代谢产物抑制了HO诱导的SH-SY5Y细胞氧化应激损伤，其机制与抑制活性氧生成、改变线粒体膜电位以及通过Nrf2/Keap1途径抑制细胞凋亡和炎症因子表达有关。
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