Department of Pharmaceutical Sciences, Central University of Haryana, Mahendergarh, India.
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, India.
Expert Opin Drug Discov. 2024 Mar;19(3):303-316. doi: 10.1080/17460441.2023.2293158. Epub 2023 Dec 19.
Owing to limited efficient treatment strategies for highly prevalent and distressing Parkinson's disease (PD), an impending need emerged for deciphering new modes and mechanisms for effective management. SH-SY5Y-based neuronal models have emerged as a new possibility for the elucidation of cellular and molecular processes in the pathogenesis of PD. SH-SY5Y cells are of human origin, adhered to catecholaminergic neuronal attributes, which consequences in imparting wide acceptance and significance to this model over conventional PD models for high-throughput screening of therapeutics.
Herein, the authors review the SH-SY5Y cell line and its value to PD research. The authors also provide the reader with their expert perspectives on how these developments can lead to the development of new impactful therapeutics.
Encouraged by recent research on SH-SY5Y cell lines, it was envisaged that this model can serve as a primary model for assessing efficacy and toxicity of new therapeutics as well as for nanocarriers' capacity in delivering therapeutic agents across BBB. Considering the proximity with human neuronal environment as in pathogenic PD conditions, SH-SY5Y cell lines vindicated consistency and reproducibility in experimental results. Accordingly, exploitation of this standardized SH-SY5Y cell line can fast-track the drug discovery and development path for novel therapeutics.
由于目前针对高发且令人痛苦的帕金森病（PD），缺乏有效的治疗策略，因此迫切需要探索新的模式和机制来进行有效管理。基于 SH-SY5Y 的神经元模型的出现为阐明 PD 发病机制中的细胞和分子过程提供了新的可能。SH-SY5Y 细胞源自人类，具有儿茶酚胺能神经元的特性，这使得该模型在高通量筛选治疗药物方面比传统的 PD 模型更广泛地被接受和重视。
本文作者回顾了 SH-SY5Y 细胞系及其在 PD 研究中的价值。作者还为读者提供了他们的专业观点，即这些进展如何能够导致新的有影响力的治疗方法的开发。
受到最近关于 SH-SY5Y 细胞系的研究的鼓舞，人们设想该模型可以作为评估新治疗药物的疗效和毒性以及纳米载体穿过血脑屏障输送治疗剂能力的主要模型。鉴于与人类神经元环境的接近性，如同在发病的 PD 条件下，SH-SY5Y 细胞系在实验结果中具有一致性和可重复性。因此，利用这种标准化的 SH-SY5Y 细胞系可以加快新型治疗药物的药物发现和开发途径。
