Mohammadi Nima, Dos Santos Lima Amanda, Azevedo Luciana, Granato Daniel
Bioactivity & Applications Lab, Department of Biological Sciences, Faculty of Science and Engineering, University of Limerick, V94 T9PX, Limerick, Ireland.
Laboratory of Nutritional and Toxicological Analyses in vitro and in vivo (LANTIN), Federal University of Alfenas, Alfenas-MG, Brazil.
Curr Res Food Sci. 2024 Mar 13;8:100714. doi: 10.1016/j.crfs.2024.100714. eCollection 2024.
Traditional antioxidant screening relies on chemical assays to assess compounds' potential in combating oxidative processes. However, translating chemical antioxidant activity to complex biological systems poses challenges. In this study, the antioxidant potential of fruit-derived phenolic compounds, hyperoside (HP), epicatechin (EC), and phlorizin (PZ), and their combinations in a specific ratio were investigated using a simplex-centroid design of experiments. The research included antioxidant assays, plasma protection against oxidation tests, and cytotoxicity assessments in human cell lines. The results revealed the complex relationship between chemical antioxidant activity and its relevance to cellular oxidative and antioxidative processes. HP and EC exhibited significant antioxidant activity, with HP outperforming EC in multiple assessments. Cytotoxicity assay confirmed that these compounds did not induce cell death or hinder proliferation, even at higher concentrations (>100 μmol/mL). In the cell antioxidant activity (CAA) test, HP and EC exhibited higher CAA, while PZ displayed lower antioxidant activity. In conclusion, a synergistic effect emerged when HP, EC, and PZ were combined, particularly in plasma protection, suggesting protective effects and potential health benefits. This research emphasized the need for a nuanced understanding of the interplay between chemical assays and cellular behavior in comprehending the relationship between chemical-based, human plasma oxidation, and CAA.
传统的抗氧化剂筛选依赖于化学分析来评估化合物对抗氧化过程的潜力。然而，将化学抗氧化活性转化到复杂的生物系统中存在挑战。在本研究中，采用单纯形重心设计实验，研究了水果衍生的酚类化合物金丝桃苷（HP）、表儿茶素（EC）和根皮苷（PZ）及其特定比例组合的抗氧化潜力。研究内容包括抗氧化分析、血浆抗氧化保护测试以及人类细胞系的细胞毒性评估。结果揭示了化学抗氧化活性与其与细胞氧化和抗氧化过程相关性之间的复杂关系。HP和EC表现出显著的抗氧化活性，在多项评估中HP优于EC。细胞毒性分析证实，即使在较高浓度（>100μmol/mL）下，这些化合物也不会诱导细胞死亡或阻碍增殖。在细胞抗氧化活性（CAA）测试中，HP和EC表现出较高的CAA，而PZ的抗氧化活性较低。总之，HP、EC和PZ组合时会产生协同效应，尤其是在血浆保护方面，表明具有保护作用和潜在的健康益处。这项研究强调，在理解基于化学的、人类血浆氧化和CAA之间的关系时，需要对化学分析与细胞行为之间的相互作用有细致入微的理解。
