Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou 310058, China.
Food Chem. 2014 Mar 1;146:363-70. doi: 10.1016/j.foodchem.2013.09.071. Epub 2013 Sep 21.
The traditional method of chemical extraction (i.e., extracts), combined with chemical antioxidant activity assays cannot assess the real antioxidant activity. In vitro digestion (i.e., digesta) with a cellular antioxidant activity (CAA) assay was developed for the determination of antioxidant activity in Chinese bayberry fruits. In this study, pretreatment methods were studied and the results showed that digesta had more free phenolic acids (FPA) but less total phenolic content (TPC) and total anthocyanin content (TAC) than extracts. Antioxidant activity assays, including ABTS, FRAP, DPPH, ORAC and CAA, were compared. Digesta had lower ABTS, FRAP and DPPH values but higher CAA values than extracts. FPA were better correlated with the chemical antioxidant assays in digesta. The correlations were high between TPC and CAA values in digesta (R(2)=0.96) but not extracts (R(2)=0.58). Higher correlations were also obtained between CAA and chemical assays in digesta.
传统的化学萃取法（即提取物）结合化学抗氧化活性测定方法不能评估真实的抗氧化活性。为了测定杨梅果实的抗氧化活性，开发了一种细胞抗氧化活性（CAA）测定的体外消化法（即消化液）。在本研究中，研究了预处理方法，结果表明，与提取物相比，消化液中游离酚酸（FPA）较多，但总酚含量（TPC）和总花青素含量（TAC）较少。比较了 ABTS、FRAP、DPPH、ORAC 和 CAA 等抗氧化活性测定方法。与提取物相比，消化液的 ABTS、FRAP 和 DPPH 值较低，但 CAA 值较高。FPA 与消化液中的化学抗氧化测定方法相关性较好。消化液中 TPC 与 CAA 值之间的相关性较高（R²=0.96），而提取物中则不高（R²=0.58）。消化液中 CAA 与化学测定方法之间也存在较高的相关性。
