表观遗传学与自杀：探究 H3K14ac 的改变揭示了 、 和 的差异表达。
Epigenetics and suicide: investigating altered H3K14ac unveiled differential expression in , and .
机构信息
Institute of Biochemistry & Molecular Genetics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Institute of Microbiology & Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
出版信息
Epigenomics. 2024;16(10):701-714. doi: 10.2217/epi-2023-0351. Epub 2024 Mar 28.
Environmental factors make an important contribution to suicide. Histone tails are prone to different modifications, leading to changes of chromatin (de)condensation and consequently gene expression. Level of H3K14ac was studied with chromatin immunoprecipitation followed by high-throughput DNA sequencing. Genes were further validated with RT-qPCR; using hippocampal tissue. We showed lowered H3K14ac levels in individuals who died by suicide. The genes , and showed differential expression in individuals who died by suicide. Identified genetic and protein interactions among genes show interactions with suicide-related genes. Further investigations of histone modifications in association with DNA methylation and miRNA are needed to expand our knowledge of the genes that could significantly contribute to suicide.
环境因素对自杀有重要贡献。组蛋白尾部容易发生不同的修饰，导致染色质（去）凝聚的变化，进而影响基因表达。通过染色质免疫沉淀和高通量 DNA 测序研究了 H3K14ac 的水平。利用海马组织的 RT-qPCR 进一步验证了基因。我们发现自杀死亡者的 H3K14ac 水平降低。基因和在自杀死亡者中表现出差异表达。鉴定出的基因间遗传和蛋白质相互作用与自杀相关基因相互作用。需要进一步研究组蛋白修饰与 DNA 甲基化和 miRNA 的关联，以扩大我们对可能对自杀有重要贡献的基因的认识。
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