Huang Lianggang, Xiao Bowen, Wang Wenjia, Li Wen, Zhang Weihong, Zhou Junping, Cai Xue, Zhang Bo, Liu Zhiqiang, Zheng Yuguo
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Zhejiang Huakang Pharmaceutical Co., Ltd., Quzhou 324302, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Mar 25;40(3):665-686. doi: 10.13345/j.cjb.230391.
Erythritol is a novel 4-carbon sugar alcohol produced by microbes in the presence of hyper-osmotic stress. It has excellent potential to serve as an alternative sugar for people with diabetes and also a platform compound for synthesizing various C4 compounds, such as 1, 3-butadiene, 1, 4-butanediol, 2, 5-dihydrofuran and so on. Compared with other polyols, the fermentative production of erythritol is more challenging. is the preferred chassis of erythritol biosynthesis for its high-titer and high-productivity. At present, there are still some bottlenecks in the production of erythritol by . , such as weak metabolic activity, abundant by-products, and low industrial attributes. Progress has been made in tailoring high version strains according to industrial needs. For example, the highest titer of erythritol produced by the metabolically engineered . reached 196 g/L and 150 g/L, respectively, by using glucose or glycerol as the carbon sources. However, further improving its production performance becomes challenging. This review summarizes the research progress in the synthesis of erythritol by . from the perspectives of erythritol producing strains, metabolic pathways, modular modifications, and auxiliary strategies to enhance the industrial properties of the engineered strain. Key nodes in the metabolic pathway and their combination strategies are discussed to guide the research on promoting the production of erythritol by . .
赤藓糖醇是微生物在高渗胁迫下产生的一种新型四碳糖醇。它具有作为糖尿病患者替代糖的巨大潜力，也是合成各种C4化合物（如1,3 - 丁二烯、1,4 - 丁二醇、2,5 - 二氢呋喃等）的平台化合物。与其他多元醇相比，赤藓糖醇的发酵生产更具挑战性。[具体菌株名称]因其高产量和高生产率而成为赤藓糖醇生物合成的首选底盘。目前，[具体菌株名称]生产赤藓糖醇仍存在一些瓶颈，如代谢活性弱、副产物多和工业属性低等问题。根据工业需求定制高版本菌株已取得进展。例如，代谢工程改造的[具体菌株名称]分别以葡萄糖或甘油为碳源时，赤藓糖醇的最高产量分别达到196 g/L和150 g/L。然而，进一步提高其生产性能具有挑战性。本综述从赤藓糖醇生产菌株、代谢途径、模块修饰以及增强工程菌株工业属性的辅助策略等方面总结了[具体菌株名称]合成赤藓糖醇的研究进展。讨论了代谢途径中的关键节点及其组合策略，以指导促进[具体菌株名称]生产赤藓糖醇的研究。
