Xu Lijuan, Zhu Siming, Wan Wenjing, Yu Ming, Zeng Xin'an, Deng Zhanhua
School of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China.
J Sci Food Agric. 2024 Aug 30;104(11):6687-6695. doi: 10.1002/jsfa.13494. Epub 2024 Apr 25.
Tangerine peel is rich in flavonoids, particularly hesperidin, which has anti-inflammatory, antioxidant and anticancer biological activities. However, it is often wasted during citrus processing. The current common extraction method for hesperidin is solvent extraction, which has the characteristics of low extraction rate and high contamination. The aim of this study was to investigate the effect of pulsed electric field-assisted alkali dissolution extraction, followed by an acidification precipitation method, on the extraction rate and structure of hesperidin from tangerine peel.
The results showed that the selected factors (material/liquid ratio, electric field intensity and pulse number) had a significant effect on the extraction yield. An optimum condition of 66.00 mL g, 4.00 kV cm and 35.00 pulses gave the maximum amount (669.38 μg mL), which was consistent with the theoretically predicted value by software (672.10 μg mL), indicating that the extraction process was feasible. In addition, the purified extract was further identified as hesperidin from UV and NMR spectra.
An appropriate strength of pulsed electric field-assisted alkali dissolution extraction followed by an acidification precipitation method can effectively improve the extraction rate of orange peel, and the purity of the extracted orange peel is higher. Compared with the traditional extraction, the pulsed electric field-assisted extraction method may be a potential technology for hesperidin extraction, which is beneficial for the high-value utilization of citrus resources. © 2024 Society of Chemical Industry.
陈皮富含黄酮类化合物，尤其是橙皮苷，其具有抗炎、抗氧化和抗癌生物活性。然而，在柑橘加工过程中，陈皮常被浪费。目前橙皮苷的常见提取方法是溶剂提取，具有提取率低和污染高的特点。本研究的目的是探讨脉冲电场辅助碱溶提取，随后酸化沉淀法对陈皮中橙皮苷提取率和结构的影响。
结果表明，所选因素（料液比、电场强度和脉冲数）对提取产率有显著影响。最佳条件为66.00 mL/g、4.00 kV/cm和35.00个脉冲时，提取量最大（669.38 μg/mL），这与软件理论预测值（672.10 μg/mL）一致，表明该提取工艺可行。此外，通过紫外光谱和核磁共振光谱进一步鉴定纯化提取物为橙皮苷。
适当强度的脉冲电场辅助碱溶提取，随后酸化沉淀法可有效提高陈皮的提取率，且提取的陈皮纯度更高。与传统提取方法相比，脉冲电场辅助提取法可能是一种潜在的橙皮苷提取技术，有利于柑橘资源的高值化利用。© 2024化学工业协会。
