Joint Institute for Food Safety and Applied Nutrition and Center for Food Safety and Security Systems, University of Maryland, College Park, Maryland, USA.
Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile.
Microbiol Spectr. 2024 May 2;12(5):e0004724. doi: 10.1128/spectrum.00047-24. Epub 2024 Mar 28.
Surface waters are considered ecological habitats where can persist and disseminate to fresh produce production systems. This study aimed to explore the genomic profiles of serotypes Typhimurium, Newport, and Infantis from surface waters in Chile, Mexico, and Brazil collected between 2019 and 2022. We analyzed the whole genomes of 106 . Typhimurium, 161 . Newport, and 113 . Infantis isolates. Our phylogenetic analysis exhibited distinct groupings of isolates by their respective countries except for a notable case involving a Chilean . Newport isolate closely related to two Mexican isolates, showing 4 and 13 single nucleotide polymorphisms of difference, respectively. The patterns of the most frequently detected antimicrobial resistance genes varied across countries and serotypes. A strong correlation existed between integron carriage and genotypic multidrug resistance (MDR) across serotypes in Chile and Mexico ( > 0.90, < 0.01), while integron(s) were not detected in any of the Brazilian isolates. By contrast, we did not identify any strong correlation between plasmid carriage and genotypic MDR across diverse countries and serotypes.IMPORTANCEUnveiling the genomic landscape of in Latin American surface waters is pivotal for ensuring public health. This investigation sheds light on the intricate genomic diversity of in surface waters across Chile, Mexico, and Brazil. Our research also addresses critical knowledge gaps, pioneering a comprehensive understanding of surface waters as a reservoir for multidrug-resistant . By integrating our understanding of integron carriage as biomarkers into broader MDR control strategies, we can also work toward targeted interventions that mitigate the emergence and dissemination of MDR in in surface waters. Given its potential implications for food safety, this study emphasizes the critical need for informed policies and collaborative initiatives to address the risks associated with in surface waters.
地表水被认为是生态栖息地， 可以在其中存活并传播到新鲜农产品生产系统中。本研究旨在探索 2019 年至 2022 年间在智利、墨西哥和巴西收集的地表水分离株 Typhimurium、Newport 和 Infantis 的基因组特征。我们分析了 106 株 Typhimurium、161 株 Newport 和 113 株 Infantis 分离株的全基因组。我们的系统发育分析表明，除了一个智利 Newport 分离株与两个墨西哥分离株密切相关的明显情况外，这些分离株按各自的国家分组。智利和墨西哥的 Newport 分离株与墨西哥的两个分离株分别有 4 和 13 个单核苷酸多态性差异，显示出明显的聚类。不同国家和血清型的最常检测到的抗生素耐药基因模式不同。在智利和墨西哥，整合子携带与基因型多药耐药（MDR）之间存在很强的相关性（ > 0.90， < 0.01），而在巴西的任何分离株中都没有检测到整合子。相比之下，我们没有发现不同国家和血清型之间质粒携带与基因型 MDR 之间存在很强的相关性。
揭示拉丁美洲地表水 中的基因组景观对于确保公共卫生至关重要。本研究揭示了智利、墨西哥和巴西地表水 中 的复杂基因组多样性。我们的研究还解决了关键的知识空白，开创了对地表水作为多药耐药 储库的全面理解。通过将我们对整合子携带作为生物标志物的理解纳入更广泛的 MDR 控制策略，我们还可以努力针对地表水 中 MDR 的出现和传播采取有针对性的干预措施。鉴于其对食品安全的潜在影响，本研究强调了制定明智政策和开展合作倡议的迫切需要，以应对地表水 相关风险。
