Cancer Research Lab, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be) University, Katraj-Dhankawadi, Pune-Satara Road, Pune-411043, Maharashtra, India.
Symbiosis School of Biological Sciences (SSBS), Symbiosis International (Deemed University), Pune, India.
J Med Microbiol. 2024 Mar;73(3). doi: 10.1099/jmm.0.001819.
Cervicovaginal diversity has been reported as a predictive biomarker for cervical cancer risk. We recently reported the bio-therapeutic potential of vaginal probiotics from healthy Indian women against vaginal pathogens, isolated from the invasive cervical cancer (ICC) patients. The cervicovaginal microflora from cervical cancer patients has not yet been reported from Indian population. The present study aimed at comparing the cervicovaginal microbiome between healthy controls (HC) and ICC patients from the Indian population. In total, 30 vaginal swabs (15 from HC and 15 from ICC) were subjected to 16S rRNA gene sequencing. Alpha diversity was evaluated by Shannon and Chao1 index; and beta diversity by principle coordinate analysis (PCoA) of weighted and unweighted UniFrac distances. The relative abundance of the microbial taxa was done according to linear discriminant analysis effect size (LEfSe). Predominance of spp. in ICC and in HC groups was observed. Alpha-diversity was found to be higher in ICC as compared to HC but was statistically non-significant. LEfSe analysis revealed and as the marker genera in ICC with a marked decrease in sp. Contrarily, in HC, and were found to be abundant. Differences in the vaginal microbiome between healthy and ICC women could help in the early prediction of cervical cancer risk and thus in designing prevention strategies.
宫颈阴道多样性已被报道为宫颈癌风险的预测生物标志物。我们最近报道了来自健康印度女性的阴道益生菌对阴道病原体的生物治疗潜力，这些病原体是从侵袭性宫颈癌（ICC）患者中分离出来的。来自印度人群的宫颈癌患者的宫颈阴道微生物群尚未报道。本研究旨在比较来自印度人群的健康对照组（HC）和 ICC 患者的宫颈阴道微生物组。总共采集了 30 个阴道拭子（15 个来自 HC，15 个来自 ICC），并进行了 16S rRNA 基因测序。通过 Shannon 和 Chao1 指数评估 alpha 多样性；通过加权和非加权 UniFrac 距离的主坐标分析（PCoA）评估 beta 多样性。根据线性判别分析效应大小（LEfSe）进行微生物分类群的相对丰度分析。在 ICC 组和 HC 组中均观察到 spp.的优势。与 HC 相比，ICC 中的 alpha 多样性较高，但统计学上无显著性差异。LEfSe 分析显示 ICC 中的 和 是标记属，而 sp. 的丰度明显降低。健康和 ICC 女性阴道微生物组之间的差异有助于早期预测宫颈癌风险，从而有助于设计预防策略。
