Department of Biochemistry, Faculty of Veterinary Medicine, University of Aydin Adnan Menderes, Aydin, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, University of Aydin Adnan Menderes, Aydin, Turkey.
Res Vet Sci. 2024 May;171:105234. doi: 10.1016/j.rvsc.2024.105234. Epub 2024 Mar 19.
This study aimed to assess the concentrations of Fibroblast Growth Factor-23 (FGF-23) and α-Klotho in healthy dogs and dogs at different stages of Canine Leishmaniasis (CanL), and investigate the changes of these parameters in relation to renal function and calcium‑phosphorus metabolism. A total of 74 dogs (22 healthy and 52 with CanL) of varying ages, sexes, and medium-sized breeds were included. Dogs with CanL were categorized into different stages (Stage I-IV) based on Leishvet recommendations. In addition to routine hematological parameters, plasma FGF-23, serum α-Klotho, urea, creatinine, phosphorus, calcium, parathormone, vitamin D concentrations, and urine protein/creatinine ratio were measured. Data from healthy dogs were compared to dogs with CanL overall and by stage. Dogs with CanL exhibited higher concentrations of FGF-23 (p < 0.05), α-Klotho, and parathormone (p < 0.001), as well as lower concentrations of vitamin D and calcium (p < 0.001). FGF-23 concentration was particularly elevated in Stage IV compared to other stages. However, no significant differences in α-Klotho levels were observed among the stages. FGF-23 levels showed a weak positive correlation with urea and creatinine concentrations and a moderate positive correlation with urine protein/creatinine ratio. This study demonstrated increased levels of FGF-23 and α-Klotho in dogs with CanL for the first time. The increase in FGF-23 levels was more prominent in advanced stages of the disease and correlated with higher urea and creatinine concentrations. These findings may serve as a basis for future diagnostic and therapeutic investigations, contributing to the understanding of the pathophysiology of kidney disease in CanL.
本研究旨在评估健康犬和不同阶段犬利什曼病（CanL）犬的成纤维细胞生长因子 23（FGF-23）和α-Klotho 浓度，并研究这些参数在肾功能和钙磷代谢方面的变化。共纳入 74 只不同年龄、性别和中型犬种的犬（22 只健康犬和 52 只患有 CanL 的犬）。根据 Leishvet 建议，将患有 CanL 的犬分为不同阶段（I-IV 期）。除了常规血液学参数外，还测量了血浆 FGF-23、血清 α-Klotho、尿素、肌酐、磷、钙、甲状旁腺激素、维生素 D 浓度和尿蛋白/肌酐比值。将健康犬的数据与整体和按阶段患有 CanL 的犬进行比较。患有 CanL 的犬的 FGF-23（p<0.05）、α-Klotho 和甲状旁腺激素（p<0.001）浓度较高，而维生素 D 和钙浓度较低（p<0.001）。与其他阶段相比，第 IV 期 FGF-23 浓度升高尤为明显。然而，各阶段的 α-Klotho 水平没有显著差异。FGF-23 水平与尿素和肌酐浓度呈弱正相关，与尿蛋白/肌酐比值呈中度正相关。本研究首次证明了患有 CanL 的犬中 FGF-23 和 α-Klotho 水平升高。在疾病的晚期阶段，FGF-23 水平的升高更为明显，与较高的尿素和肌酐浓度相关。这些发现可能为未来的诊断和治疗研究提供依据，有助于理解 CanL 中肾脏疾病的病理生理学。
