Department of Blood Transfusion, The first Medical Center, Chinese PLA General Hospital, Beijing, 100853, China.
Department of Blood Transfusion, The first Medical Center, Chinese PLA General Hospital, Beijing, 100853, China.
Diagn Microbiol Infect Dis. 2024 Jun;109(2):116240. doi: 10.1016/j.diagmicrobio.2024.116240. Epub 2024 Mar 2.
Occult HBV infection (OBI) remains a potential threat for blood safety. The prevalence of OBI was investigated in a blood donation center of Chinese PLA General Hospital to improve HBV blood safety. 229446 samples from blood donors were screened by two different enzyme-linked immunosorbent assay (ELISA) kits. 78 samples were HBV DNA positive among 212134 ELISA nonreactive donor samples. The prevalence of OBI was 0.04% (76/212134). Ten samples of OBI were permitted by the donors' content for further research, and all of these were below 200IU/mL, and six of these were below 20IU/mL(6/10,60%). Genotype B and genotype C was 20% (2/10) and 80% (8/10), respectively. 16 amino acid mutations were detected in the S region of OBI, included three mutations in MHR region of S. The prevalence of OBI is rare in this donation center. These mutations we found may contribute to the multifactorial occurrence of OBI.
隐匿性乙型肝炎病毒感染（OBI）仍然是血液安全的潜在威胁。为了提高乙型肝炎病毒血液安全性，本研究在解放军总医院献血中心调查了 OBI 的流行情况。采用两种不同的酶联免疫吸附试验（ELISA）试剂盒对 229446 名献血者样本进行筛查。在 212134 例 ELISA 非反应性供者样本中，78 例 HBV DNA 阳性。OBI 的流行率为 0.04%（76/212134）。10 份 OBI 样本经供者同意进行进一步研究，所有样本均低于 200IU/mL，其中 6 份样本低于 20IU/mL（6/10，60%）。基因型 B 和基因型 C 分别占 20%（2/10）和 80%（8/10）。在 OBI 的 S 区检测到 16 个氨基酸突变，包括 S 区 MHR 区域的 3 个突变。在该献血中心，OBI 的流行率罕见。我们发现的这些突变可能导致 OBI 的多因素发生。
