Laboratório de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, SP, Brazil.
Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo, São José dos Campos, SP, Brazil.
Methods Mol Biol. 2024;2758:319-329. doi: 10.1007/978-1-0716-3646-6_17.
Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here, we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.
蛇毒肽组被认为是具有不同药理特性的分子的重要来源。蛇毒的复杂性和变异性、蛋白酶的存在以及缺乏完整的特定物种基因组序列，使得蛇毒肽组谱分析成为一项具有挑战性的任务，需要特别的技术策略来进行样品处理和质谱分析。在这里，我们描述了一种评估蛇毒肽含量的方法，并强调了采样程序的重要性，因为它们会极大地影响蛇毒的肽组复杂性。
