College of Life Science, Henan Normal University, Xinxiang 453007, China.
Henan International Joint Laboratory of Agricultural Microbial Ecology and Technology, Henan Normal University, Xinxiang 453007, China.
Plant Dis. 2024 Aug;108(8):2472-2483. doi: 10.1094/PDIS-01-24-0169-RE. Epub 2024 Aug 2.
Yam is an important medicinal and edible dual-purpose plant with high economic value. However, nematode damage severely affects its yield and quality. One of the major effects of nematode infestations is the secondary infection of pathogenic bacteria or fungi through entry wounds made by the nematodes. Understanding the response of the symbiotic microbial community of yam plants to nematodes is crucial for controlling such a disease. In this study, we investigated the rhizosphere and how endophytic microbiomes shift after nematode infection during the tuber expansion stage in the Thunb. cultivar Tiegun. Our results revealed that soil depth affected the abundance of nematodes, and the relative number of was higher in the diseased soil at a depth of 16 to 40 cm than those at a depth of 0 to 15 and 41 to 70 cm. The abundance of and interactions among soil microbiota members were significantly correlated with root-knot nematode (RKN) parasitism at various soil depths. However, the comparison of the microbial α-diversity and composition between healthy and diseased rhizosphere soil showed no difference. Compared with healthy soils, the co-occurrence networks of -infested soils included a higher ratio of positive correlations linked to plant health. In addition, we detected a higher abundance of certain taxonomic groups belonging to Chitinophagaceae and Xanthobacteraceae in the rhizosphere of RKN-infested plants. The nematodes, besides causing direct damage to plants, also possess the ability to act synergistically with other pathogens, especially and , leading to the development of disease complexes. In contrast to soil samples, RKN parasitism specifically had a significant effect on the composition and assembly of the root endophytic microbiota. The RKN colonization impacted a wide variety of endophytic microbiomes, including , , , , and . This study revealed the relationship between RKN disease and changes in the rhizosphere and endophytic microbial community, which may provide novel insights that help improve biological management of yam RKNs.
山药是一种重要的药食两用植物，具有很高的经济价值。然而，线虫的侵害严重影响了它的产量和质量。线虫侵染的主要影响之一是通过线虫造成的入口伤口，使病原细菌或真菌再次感染。了解山药植物共生微生物群落对线虫的反应对于控制这种疾病至关重要。在这项研究中，我们研究了根际和块茎扩张阶段线虫感染后内生微生物组的变化，供试品种为 Thunb. cultivar Tiegun。我们的结果表明，土壤深度影响线虫的丰度，在 16 到 40 厘米深的患病土壤中， 的相对数量高于 0 到 15 厘米和 41 到 70 厘米深的土壤。土壤微生物群成员的丰度和相互作用与不同土壤深度的根结线虫（RKN）寄生密切相关。然而，健康和患病根际土壤之间微生物 α 多样性和组成的比较没有差异。与健康土壤相比，受 -侵染土壤的共生网络包含更高比例与植物健康相关的正相关关系。此外，我们在 RKN 侵染植物的根际中检测到某些分类群的丰度较高，属于几丁质分解菌科和黄杆菌科。线虫除了直接对植物造成损害外，还具有与其他病原体协同作用的能力，特别是 和 ，导致疾病复合体的发展。与土壤样本不同，RKN 寄生对线根内生微生物群落的组成和组装有显著影响。RKN 定植影响了广泛的内生微生物组，包括 、 、 、 、和 。这项研究揭示了 RKN 疾病与根际和内生微生物群落变化之间的关系，这可能为改善山药 RKN 的生物管理提供新的见解。
