School of Life Sciences, Anhui University, Hefei 230601, China.
Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei 230601, China.
J Appl Microbiol. 2024 Sep 2;135(9). doi: 10.1093/jambio/lxae218.
The objective of this study was to elucidate the role and mechanism of changes in the rhizosphere microbiome following Arthrobotrys oligospora treatment in the biological control of root-knot nematodes and identify the key fungal and bacterial species that collaborate with A. oligospora to biocontrol root-knot nematodes.
We conducted a pot experiment to investigate the impact of A. oligospora treatment on the biocontrol efficiency of A. oligospora against Meloidogyne incognita infecting tomatoes. We analyzed the rhizosphere bacteria and fungi communities of tomato by high-throughput sequencing of the 16S rRNA gene fragment and the internal transcribed spacer (ITS). The results indicated that the application of A. oligospora resulted in a 53.6% reduction in the disease index of M. incognita infecting tomato plants. The bacterial diversity of rhizosphere soil declined in the A. oligospora-treated group, while fungal diversity increased. The A. oligospora treatment enriched the tomato rhizosphere with Acidobacteriota, Firmicutes, Bradyrhizobium, Sphingomonadales, Glomeromycota, and Purpureocillium. These organisms are involved in the utilization of rhizosphere organic matter, nitrogen, and glycerolipids, or play the role of ectomycorrhiza or directly kill nematodes. The networks of bacterial and fungal co-occurrence exhibited a greater degree of stability and complexity in the A. oligospora treatment group.
This study demonstrated the key fungal and bacterial species that collaborate with the A. oligospora in controlling the root-knot nematode and elaborated the potential mechanisms involved. The findings offer valuable insights and inspiration for the advancement of bionematicide based on nematode-trapping fungi.
本研究旨在阐明寡枝腐霉处理后根际微生物组的变化及其在根结线虫生物防治中的作用和机制，并鉴定与寡枝腐霉协同防治根结线虫的关键真菌和细菌物种。
我们进行了盆栽试验，以研究寡枝腐霉处理对其防治南方根结线虫（Meloidogyne incognita）感染番茄的生物防治效率的影响。通过高通量测序分析了番茄根际细菌和真菌群落的 16S rRNA 基因片段和内部转录间隔区（ITS）。结果表明，应用寡枝腐霉可使南方根结线虫感染番茄的病指降低 53.6%。寡枝腐霉处理组根际土壤细菌多样性下降，而真菌多样性增加。寡枝腐霉处理增加了根际土壤中的 Acidobacteriota、Firmicutes、Bradyrhizobium、Sphingomonadales、Glomeromycota 和 Purpureocillium。这些生物参与了根际有机质、氮和甘油脂的利用，或发挥着外生菌根或直接杀死线虫的作用。细菌和真菌共发生网络在寡枝腐霉处理组中表现出更高的稳定性和复杂性。
本研究鉴定了与寡枝腐霉协同防治根结线虫的关键真菌和细菌物种，并阐述了其潜在的作用机制。这些发现为基于线虫诱捕真菌的生物杀线虫剂的发展提供了有价值的见解和启示。
