C 型利钠肽对改善伊拉克本地公羊附睾精子的效率。

Efficiency of C-type natriuretic peptide on improvement of Iraqi local ram's epididymal sperms.

机构信息

Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.

出版信息

Open Vet J. 2024 Feb;14(2):674-682. doi: 10.5455/OVJ.2024.v14.i2.7. Epub 2024 Feb 29.

DOI:10.5455/OVJ.2024.v14.i2.7

PMID:38549573

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10970128/

Abstract

BACKGROUND

Fertility plays a great role in animal reproduction since high-quality semen improves sheep industry reproduction. The current worldwide data revealed the close relation of C-type natriuretic peptide (CNP) to the reproductive function of rams.

AIMS

Evaluation of the effect of CNP on cooled sperms using traditional and molecular assays.

METHODS

Totally, of 20 testicular samples were collected, processed to obtain the semen samples, and divided into two parts; one was treated with a suitable dose of CNP, and the other served as a control. Sperm samples of both groups were cooled for 3 days and tested at 0, 24, 48, and 72 hours.

RESULTS

The findings revealed that the suitable dose of CNP-treated sperms was 0.01 × 10-. Values of individual motility, live sperms, and sperm concentration were reduced significantly in CNP-24h, CNP-48h, and CNP-72h when compared to control; however, abnormal sperms were increased in both control and CNP groups at 24, 48, and 72 hours when compared to values of 0 hour. Concerning turbidmetric analysis, a significant reduction in values of lag time was observed in CNP when compared to control at all times of cooling intervals. In both CNP and control groups, motility index was decreased at 24, 48, and 72 hours when compared to 0 hour. For velocity, significant increases were shown in CNP compared with control at all cooling intervals. However, values of both groups were increased significantly at 24, 48, and 72 hours times when compared to 0 hour. Fraction of rapidly moving sperm of CNP was elevated at 0 hour and decreased at 24, 48, and 72 hours when compared to control. Expression of the gene was reduced gradually in sperms of CNP and control groups at times of cooling intervals.

CONCLUSION

To the best of our knowledge, this first Iraqi study targets the effect of CNP on epididymal sperms of rams. However, changes that occur after excessive CNP exposure remain unclear, and the toxicological profile of CNP requires furthermore supplements.

摘要

背景

在动物繁殖中，生育力起着重要作用，因为高质量的精液可以提高绵羊养殖业的繁殖能力。目前全球数据显示，C 型利钠肽（CNP）与公羊的生殖功能密切相关。

目的

使用传统和分子方法评估 CNP 对冷却精子的影响。

方法

总共收集了 20 个睾丸样本，进行处理以获得精液样本，并将其分为两部分；一部分用适当剂量的 CNP 处理，另一部分作为对照。两组的精子样本均冷却 3 天，并在 0、24、48 和 72 小时进行测试。

结果

研究结果表明，CNP 处理精子的适宜剂量为 0.01×10-6。与对照组相比，CNP-24h、CNP-48h 和 CNP-72h 时个体活力、活精子和精子浓度显著降低；然而，在 24、48 和 72 小时时，与 0 小时的数值相比，对照组和 CNP 组的异常精子数量均增加。关于浊度分析，与对照组相比，CNP 在所有冷却间隔时间的滞后时间值均显著降低。在 CNP 和对照组中，与 0 小时相比，在 24、48 和 72 小时时运动指数均降低。对于速度，与对照组相比，在所有冷却间隔时间内，CNP 都显示出显著增加。然而，与 0 小时相比，两组在 24、48 和 72 小时时的值均显著增加。CNP 的快速运动精子比例在 0 小时时升高，在 24、48 和 72 小时时降低，与对照组相比。在冷却间隔时间内，CNP 和对照组精子中的基因表达逐渐降低。