Department of Veterinary Science, Veterinary Medicine Faculty, Airlangga University, Mulyorejo, Surabaya, East Java, Indonesia.
Department of Chemistry, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
Open Vet J. 2024 Feb;14(2):738-742. doi: 10.5455/OVJ.2024.v14.i2.14. Epub 2024 Feb 29.
Chronic physical stress has many effects on the nervous system and can cause structural changes in different parts of the brain and hemomodulatory, including hormonal. Current pharmacotherapeutic treatments have limited efficacy and are associated with many deleterious side effects.
The aim of this research is to determine how forest honey administration affects follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels in rats who are subjected to forced swim tests as a model of chronic physical stress placed in a container filled with water from which it cannot escape.
This was an experimental laboratory study with 32 rats divided into four treatment groups: control (C), Treatment 1 (T1) with a forced swim test + honey (2 g/rat/day), Treatment 2 (T2) with a forced swim test + honey (4 g/rat/day), and Treatment 3 (T3) with a forced swim test + honey (6 g/rat/day). All treatments were administered for 14 days. Then, blood was taken for FSH and LH serum tests, and a one-way ANOVA and Duncan test were used to statistically test the data analysis.
The results of this study indicate that the administration of forest honey had no significant effect ( > 0.05) on the FSH parameter, but there was a significant decrease in LH levels in the T2 and T3 groups ( < 0.05).
It can be concluded that giving forest honey to rats who were subjected to a 14-day forced swim test had no effect on FSH and LH levels. In rats given a forced swim test as a model of chronic stress, administration at doses of 4 and 6 g/rat/day reduced LH serum levels. Thus, giving forest honey could maintain reproductive health in rat that experience chronic stress.
慢性身体应激对神经系统有许多影响，并会导致大脑不同部位的结构变化和血液调节，包括激素。目前的药物治疗疗效有限，且伴有许多有害的副作用。
本研究旨在确定在作为慢性身体应激模型的强迫游泳试验中，给大鼠施用森林蜂蜜会如何影响促卵泡激素（FSH）和黄体生成素（LH）水平。大鼠被放置在一个装满水的容器中，它们无法从中逃脱。
这是一项实验性实验室研究，共有 32 只大鼠分为 4 个治疗组：对照组（C）、有强迫游泳试验+蜂蜜（2 g/大鼠/天）的治疗 1 组（T1）、有强迫游泳试验+蜂蜜（4 g/大鼠/天）的治疗 2 组（T2）和有强迫游泳试验+蜂蜜（6 g/大鼠/天）的治疗 3 组（T3）。所有治疗均持续 14 天。然后，采集血液进行 FSH 和 LH 血清检测，采用单因素方差分析和 Duncan 检验对数据进行统计学分析。
本研究结果表明，森林蜂蜜给药对 FSH 参数没有显著影响（>0.05），但 T2 和 T3 组的 LH 水平显著下降（<0.05）。
可以得出结论，给经历了 14 天强迫游泳试验的大鼠施用森林蜂蜜对 FSH 和 LH 水平没有影响。在作为慢性应激模型的大鼠中，给予 4 和 6 g/大鼠/天剂量的蜂蜜可降低 LH 血清水平。因此，给大鼠施用森林蜂蜜可以维持其在慢性应激下的生殖健康。
