Jian Rui, Xue Jing, Xu Ze-Yun, Chen Si-Si, Wang Fang-Ni, Du Luanying, Xie Guang-Cheng, Guo Wen-Ping
Department of Pathogenic Biology, College of Basic Medicine, Chengde Medical University, Chengde, Hebei, China.
Front Vet Sci. 2024 Mar 14;11:1373178. doi: 10.3389/fvets.2024.1373178. eCollection 2024.
Dogs and cats are the hosts of many vector-borne human pathogens that can be transmitted to humans. Given their direct and intimate contact with humans, companion dogs and cats are considered direct sentinels of vector-borne human pathogens. However, limited information is currently available regarding canine and feline zoonotic pathogens in China. This study detected canine and feline vector-borne human pathogens to better understand the potential risk to humans.
Blood samples were collected from 275 domestic companion animals (117 dogs and 158 cats) living in Tianjin city, China, and the presence of DNA from , , , and was detected by semi-nested polymerase chain reaction (PCR). The PCR products of the expected size were sequenced, and these newly generated sequences were subjected to BLASTN, nucleotide identity, and phylogenetic analyses.
A total of 24 blood samples tested positive for vector-borne pathogens in companion dogs and cats in Tianjin city, China, with a relatively low positive rate of 8.7%. Specifically, seven human pathogens, including , , , , , , and , were identified. In addition, with zoonotic potential and were detected.
Our results indicate substantial genetic diversity in the vector-borne human pathogens circulating in companion dogs and cats. Interventions based on "One Health" should be taken to reduce the potential risks of contracting infection from companion dogs and cats in Tianjin, China.
犬类和猫类是许多可传播给人类的媒介传播人类病原体的宿主。鉴于它们与人类的直接密切接触，伴侣犬和猫被视为媒介传播人类病原体的直接哨兵。然而，目前关于中国犬类和猫类人畜共患病原体的信息有限。本研究检测犬类和猫类媒介传播的人类病原体，以更好地了解对人类的潜在风险。
从中国天津市的275只家养伴侣动物（117只狗和158只猫）采集血样，通过半巢式聚合酶链反应（PCR）检测 、 、 和 的DNA存在情况。对预期大小的PCR产物进行测序，并对这些新生成的序列进行BLASTN、核苷酸同一性和系统发育分析。
在中国天津市，共有24份伴侣犬和猫的血样检测出媒介传播病原体呈阳性，阳性率相对较低，为8.7%。具体而言，鉴定出七种人类病原体，包括 、 、 、 、 、 和 。此外，还检测到具有人畜共患病潜力的 和 。
我们的结果表明，在伴侣犬和猫中传播的媒介传播人类病原体存在大量遗传多样性。应采取基于“同一健康”的干预措施，以降低中国天津市从伴侣犬和猫感染疾病的潜在风险。
