Testa J R, Misawa S, Oguma N, Van Sloten K, Wiernik P H
Cancer Res. 1985 Jan;45(1):430-4.
Cytogenetic studies, using improved short-term culture techniques, were performed on 64 patients with acute leukemia to determine the incidence and kinds of clonal karyotypic changes detectable with this newer methodology. An adequate number of analyzable mitoses was obtained from 59 patients. Clonal chromosomal alterations were found in 88% (52 of 59) of patients, as compared to approximately 50% in previous studies of acute leukemia in which conventional techniques were used. From our series, abnormal karyotypes were detected in 37 of 44 (84%) cases with primary acute nonlymphocytic leukemia, all 5 with secondary acute nonlymphocytic leukemia, and all 10 with acute lymphoblastic leukemia. Among the entire group of patients, several recurrent abnormalities were observed, e.g., -7 in eight cases, +8 in seven cases, t(15;17) in four cases, and t(8;21) or a variant of this translocation in four cases. In five patients, the only abnormality was a rather subtle structural rearrangement (e.g., tiny deletion). Five other patients had clonal changes which were found in less than 10% of the mitoses examined in each case. Our results indicate that most patients with acute leukemia, both acute nonlymphocytic leukemia and acute lymphoblastic leukemia, have clonal chromosome abnormalities associated with their disease.
采用改良的短期培养技术，对64例急性白血病患者进行了细胞遗传学研究，以确定使用这种更新方法可检测到的克隆性核型改变的发生率和种类。从59例患者中获得了足够数量的可分析有丝分裂细胞。88%（59例中的52例）的患者发现了克隆性染色体改变，而以往使用传统技术研究急性白血病时这一比例约为50%。在我们的研究系列中，44例原发性急性非淋巴细胞白血病患者中有37例（84%）检测到异常核型，5例继发性急性非淋巴细胞白血病患者均检测到异常核型，10例急性淋巴细胞白血病患者也均检测到异常核型。在整个患者组中，观察到了几种复发性异常，例如，8例患者出现-7，7例患者出现+8，4例患者出现t(15;17)，4例患者出现t(8;21)或该易位的变异型。5例患者唯一的异常是一种相当细微的结构重排（例如微小缺失）。另外5例患者的克隆性改变在每个病例中所检查的有丝分裂细胞中不到10%。我们的结果表明，大多数急性白血病患者，包括急性非淋巴细胞白血病和急性淋巴细胞白血病患者，都有与其疾病相关的克隆性染色体异常。
