Engineering Lab of Henan Province for Aquatic Animal Disease Control, Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, Xinxiang, 453007, PR China; Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, Nanyang, 474450, Henan, China.
Engineering Lab of Henan Province for Aquatic Animal Disease Control, Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, Xinxiang, 453007, PR China.
Fish Shellfish Immunol. 2024 May;148:109521. doi: 10.1016/j.fsi.2024.109521. Epub 2024 Mar 27.
In mammals, β-catenin participates in innate immune process through interaction with NF-κB signaling pathway. However, its role in teleost immune processes remains largely unknown. We aimed to clarify the function of β-catenin in the natural defense mechanism of Qi river crucian carp (Carassius auratus). β-catenin exhibited a ubiquitous expression pattern in adult fish, as indicated by real-time PCR analysis. Following lipopolysaccharide (LPS), Polyinosinic-polycytidylic acid (polyI: C) and Aeromonas hydrophila (A. hydrophila) challenges, β-catenin increased in gill, intestine, liver and kidney, indicating that β-catenin likely plays a pivotal role in the immune response against pathogen infiltration. Inhibition of the β-catenin pathway using FH535, an inhibitor of Wnt/β-catenin pathway, resulting in pathological damage of the gill, intestine, liver and kidney, significant decrease of innate immune factors (C3, defb3, LYZ-C, INF-γ), upregulation of inflammatory factors (NF-κB, TNF-α, IL-1, IL-8), and downregulation of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activities, increase of Malondialdehyde (MDA) content. Following A. hydrophila invasion, the mortality rate in the FH535 treatment group exceeded that of the control group. In addition, the diversity of intestinal microflora decreased and the community structure was uneven after FH535 treatment. In summary, our findings strongly suggest that β-catenin plays a vital role in combating pathogen invasion and regulating intestinal flora in Qi river crucian carp.
在哺乳动物中，β-连环蛋白通过与 NF-κB 信号通路相互作用参与固有免疫过程。然而，其在硬骨鱼类免疫过程中的作用在很大程度上尚不清楚。本研究旨在阐明β-连环蛋白在淇河鲫（Carassius auratus）天然防御机制中的功能。实时 PCR 分析表明，β-连环蛋白在成年鱼中呈普遍表达模式。在受到脂多糖（LPS）、聚肌胞苷酸（polyI: C）和嗜水气单胞菌（A. hydrophila）的刺激后，β-连环蛋白在鳃、肠、肝和肾中增加，表明β-连环蛋白可能在抵御病原体入侵的免疫反应中发挥关键作用。使用 Wnt/β-连环蛋白通路抑制剂 FH535 抑制β-连环蛋白通路，导致鳃、肠、肝和肾出现病理损伤，固有免疫因子（C3、defb3、LYZ-C、INF-γ）显著减少，炎症因子（NF-κB、TNF-α、IL-1、IL-8）上调，谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性降低，丙二醛（MDA）含量增加。在受到嗜水气单胞菌侵袭后，FH535 处理组的死亡率超过对照组。此外，FH535 处理后肠道微生物群落的多样性降低，群落结构不均匀。综上所述，本研究结果强烈表明β-连环蛋白在淇河鲫抵御病原体入侵和调节肠道菌群方面发挥着重要作用。
