Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, 603203, India.
Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology- Ramapuram, Chennai, Tamil Nadu, 600089, India.
Med Oncol. 2024 Mar 29;41(5):103. doi: 10.1007/s12032-024-02341-5.
Rauvolfia tetraphylla is an essential medicinal plant that has been widely used in traditional medicine for various disease treatments. However, the tumor suppressor activity of R. tetraphylla and its phytocompounds were not explored against triple-negative breast cancer. The current research investigated the impact of R. tetraphylla methanolic extract (RTE) and its isolated compounds Ajmaline (RTC1) and Reserpine (RTC2) on triple-negative breast cancer cell line (MDA-MB-231) focusing on anti-proliferative effects. Our study imparts that RTE and RTC2 showed promising cytotoxic effects compared to RTC1. So further experiments have proceeded with RTE and RTC2, to evaluate its proliferation, migration, and apoptotic effect. The result shows around 80% of cells were observed in the G0/G1 phase in cell cycle analysis indicating the cell cycle inhibition and duel staining clearly showed the apoptotic effect. The migration of cells after the scratch was 60.45% observed in control and 90% in treated cells showing the inhibition of migration. ROS distribution was intense compared to control indicating the increased ROS stress in treated cells. Both RTE and RTC2-treated cells showed the potential to suppress proliferation and induce apoptotic change by upregulating BAX and MST-1 and suppressing Bcl2, LATS-1, and YAP, proving that deregulation of YAP resulting in the blockage of TEAD-YAP complex and inhibit proliferation. Therefore, R. tetraphylla extract and its isolated compounds were demonstrated to find its ability to act against MDA-MB-231 and these findings will help adjudicate it as a therapeutic drug against experimental triple-negative breast cancer.
萝芙木是一种重要的药用植物，在传统医学中被广泛用于治疗各种疾病。然而，萝芙木及其植物化合物的肿瘤抑制活性尚未在三阴性乳腺癌中得到探索。本研究旨在探讨萝芙木甲醇提取物（RTE）及其分离化合物阿马林（RTC1）和利血平（RTC2）对三阴性乳腺癌细胞系（MDA-MB-231）的影响，重点研究其抗增殖作用。我们的研究表明，RTE 和 RTC2 比 RTC1 显示出有希望的细胞毒性作用。因此，我们进一步用 RTE 和 RTC2 进行了实验，以评估其增殖、迁移和凋亡作用。结果表明，细胞周期分析中约有 80%的细胞处于 G0/G1 期，表明细胞周期抑制和双重染色清楚地显示了凋亡作用。划痕后细胞的迁移率在对照组中观察到 60.45%，而在处理组中观察到 90%，表明迁移受到抑制。与对照组相比，ROS 分布更加密集，表明处理细胞中的 ROS 应激增加。RTE 和 RTC2 处理的细胞均表现出通过上调 BAX 和 MST-1 以及抑制 Bcl2、LATS-1 和 YAP 来抑制增殖和诱导凋亡变化的潜力，证明 YAP 的失调导致 TEAD-YAP 复合物的阻断并抑制增殖。因此，萝芙木提取物及其分离化合物被证明具有针对 MDA-MB-231 的作用能力，这些发现将有助于将其作为治疗实验性三阴性乳腺癌的药物。
